Expression of vitamin D receptor in bronchial asthma and its bioinformatics prediction.

ABSTRACT: Vitamin D receptors (VDRs) are associated with the occurrence and development of asthma. The aim of the present study was to analyze the secondary structure and B‑cell and T‑cell epitopes of VDR using online prediction software and aid in the future development of a highly efficient epitope‑based vaccine against asthma. Blood samples were collected from peripheral blood of asthmatic children. Reverse transcription quantitative‑polymerase chain reaction (RT‑qPCR) was performed to detect the expression of VDR in the peripheral blood. Mouse models of asthma were established. Hematoxylin and eosin staining was performed to observe the pathological alterations of the lungs of mice. Immunohistochemistry, western blot analysis and RT‑qPCR were performed to detect the expression of VDR in the lung

SUBMITTER: Shan L 

PROVIDER: S-EPMC6072178 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature