Dataset Information

BAS2 Is Required for Conidiation and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis.

ABSTRACT: The hemibiotrophic fungi Colletotrichum gloeosporioides can cause anthracnose in rubber trees. By searching the genome of the fungal pathogen, the BAS2 encoding a biotrophy-associated secreted protein was identified. In the present study, the knockout mutants of BAS2 were constructed and the functions of BAS2 were investigated. The in vitro assays showed that BAS2 was not necessary for vegetative growth but was important for normal asexual reproduction in C. gloeosporioides. Pathogenicity assays suggested that BAS2 was involved in the process of the pathogen penetrating into the host tissue. Subcellular localization analysis revealed that BAS2 showed secretional characteristics in the fungi, and BAS2 mainly function as a cytoplasmic protein after being secreted into the host cell. Extracellular proteomics analysis revealed that BAS2 was required for the secretion of a series of proteins, which were important for the pathogenicity of C. gloeosporioides. These data lead to a better understanding of the biotrophy-associated secreted protein in regulating the pathogenesis of C. gloeosporioides.

SUBMITTER: An B

PROVIDER: S-EPMC6073657 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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BAS2 Is Required for Conidiation and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis.

An Bang B Wang Wenfeng W Guo Yunfeng Y Wang Qiannan Q Luo Hongli H He Chaozu C

International journal of molecular sciences 20180625 7

The hemibiotrophic fungi Colletotrichum gloeosporioides can cause anthracnose in rubber trees. By searching the genome of the fungal pathogen, the BAS2 encoding a biotrophy-associated secreted protein was identified. In the present study, the knockout mutants of BAS2 were constructed and the functions of BAS2 were investigated. The in vitro assays showed that BAS2 was not necessary for vegetative growth but was important for normal asexual reproduction in C. gloeos ...[more]

PMID: 29941774

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Project description:The limit of the Colletotrichum gloeosporioides species complex is defined genetically, based on a strongly supported clade within the Colletotrichum ITS gene tree. All taxa accepted within this clade are morphologically more or less typical of the broadly defined C. gloeosporioides, as it has been applied in the literature for the past 50 years. We accept 22 species plus one subspecies within the C. gloeosporioides complex. These include C. asianum, C. cordylinicola, C. fructicola, C. gloeosporioides, C. horii, C. kahawae subsp. kahawae, C. musae, C. nupharicola, C. psidii, C. siamense, C. theobromicola, C. tropicale, and C. xanthorrhoeae, along with the taxa described here as new, C. aenigma, C. aeschynomenes, C. alatae, C. alienum, C. aotearoa, C. clidemiae, C. kahawae subsp. ciggaro, C. salsolae, and C. ti, plus the nom. nov. C. queenslandicum (for C. gloeosporioides var. minus). All of the taxa are defined genetically on the basis of multi-gene phylogenies. Brief morphological descriptions are provided for species where no modern description is available. Many of the species are unable to be reliably distinguished using ITS, the official barcoding gene for fungi. Particularly problematic are a set of species genetically close to C. musae and another set of species genetically close to C. kahawae, referred to here as the Musae clade and the Kahawae clade, respectively. Each clade contains several species that are phylogenetically well supported in multi-gene analyses, but within the clades branch lengths are short because of the small number of phylogenetically informative characters, and in a few cases individual gene trees are incongruent. Some single genes or combinations of genes, such as glyceraldehyde-3-phosphate dehydrogenase and glutamine synthetase, can be used to reliably distinguish most taxa and will need to be developed as secondary barcodes for species level identification, which is important because many of these fungi are of biosecurity significance. In addition to the accepted species, notes are provided for names where a possible close relationship with C. gloeosporioides sensu lato has been suggested in the recent literature, along with all subspecific taxa and formae speciales within C. gloeosporioides and its putative teleomorph Glomerella cingulata.Taxonomic noveltiesName replacement - C. queenslandicum B. Weir & P.R. Johnst. New species - C. aenigma B. Weir & P.R. Johnst., C. aeschynomenes B. Weir & P.R. Johnst., C. alatae B. Weir & P.R. Johnst., C. alienum B. Weir & P.R. Johnst, C. aotearoa B. Weir & P.R. Johnst., C. clidemiae B. Weir & P.R. Johnst., C. salsolae B. Weir & P.R. Johnst., C. ti B. Weir & P.R. Johnst. New subspecies - C. kahawae subsp. ciggaro B. Weir & P.R. Johnst. Typification: Epitypification - C. queenslandicum B. Weir & P.R. Johnst.

Dataset Information

BAS2 Is Required for Conidiation and Pathogenicity of Colletotrichum gloeosporioides from Hevea brasiliensis.

Publications

BAS2 Is Required for Conidiation and Pathogenicity of <i>Colletotrichum gloeosporioides</i> from <i>Hevea brasiliensis</i>.

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