Dataset Information

Association between Y-Maze Acquisition Learning and Major Histocompatibility Complex Class II Polymorphisms in Mice.

ABSTRACT: Objective:To explore the association between the acquisition process in the Y-maze and H-2 class II polymorphisms in mice. Methods:Mice were trained for 5 consecutive days in the Y-maze. The value of the slope of the latent period was considered an indication for the acquisition process. A slope < 0 indicated learning during the training and a slope > 0 indicated no learning. The H-2 polymorphism was determined with PCR amplification, and the correlation between the alleles and the acquisition process was analyzed. Results:The overall percentage of mice that learned was 46.1%. The percentage of mice that had learned with MudoEb5 (37.9%) was significantly lower than that of mice without MudoEb5 (61.1%; P < 0.05). The percentage of mice that had learned with MudoEb7 (26.1%) was significantly lower than that of mice without MudoEb7 (51.9%; P < 0.05). Conclusions:The major histocompatibility complex (MHC) and other alleles may be involved in the acquisition process. There may be a biological basis for learning in mice.

SUBMITTER: Ru M

PROVIDER: S-EPMC6077659 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Association between Y-Maze Acquisition Learning and Major Histocompatibility Complex Class II Polymorphisms in Mice.

Ru Ming M Liu Hui H

BioMed research international 20180719

<h4>Objective</h4>To explore the association between the acquisition process in the Y-maze and H-2 class II polymorphisms in mice.<h4>Methods</h4>Mice were trained for 5 consecutive days in the Y-maze. The value of the slope of the latent period was considered an indication for the acquisition process. A slope < 0 indicated learning during the training and a slope > 0 indicated no learning. The H-2 polymorphism was determined with PCR amplification, and the correlation between the alleles and th ...[more]

PMID: 30112411

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Project description:Sirenians share with cetaceans and pinnipeds several convergent traits selected for the aquatic lifestyle. Living in water poses new challenges not only for locomotion and feeding but also for combating new pathogens, which may render the immune system one of the best tools aquatic mammals have for dealing with aquatic microbial threats. So far, only cetaceans have had their class II Major Histocompatibility Complex (MHC) organization characterized, despite the importance of MHC genes for adaptive immune responses. This study aims to characterize the organization of the marine mammal class II MHC using publicly available genomes. We located class II sequences in the genomes of one sirenian, four pinnipeds and eight cetaceans using NCBI-BLAST and reannotated the sequences using local BLAST search with exon and intron libraries. Scaffolds containing class II sequences were compared using dotplot analysis and introns were used for phylogenetic analysis. The manatee class II region shares overall synteny with other mammals, however most DR loci were translocated from the canonical location, past the extended class II region. Detailed analysis of the genomes of closely related taxa revealed that this presumed translocation is shared with all other living afrotherians. Other presumptive chromosome rearrangements in Afrotheria are the deletion of DQ loci in Afrosoricida and deletion of DP in E. telfairi. Pinnipeds share the main features of dog MHC: lack of a functional pair of DPA/DPB genes and inverted DRB locus between DQ and DO subregions. All cetaceans share the Cetartiodactyla inversion separating class II genes into two subregions: class IIa, with DR and DQ genes, and class IIb, with non-classic genes and a DRB pseudogene. These results point to three distinct and unheralded class II MHC structures in marine mammals: one canonical organization but lacking DP genes in pinnipeds; one bearing an inversion separating IIa and IIb subregions lacking DP genes found in cetaceans; and one with a translocation separating the most diverse class II gene from the MHC found in afrotherians and presumptive functional DR, DQ, and DP genes. Future functional research will reveal how these aquatic mammals cope with pathogen pressures with these divergent MHC organizations.

Dataset Information

Association between Y-Maze Acquisition Learning and Major Histocompatibility Complex Class II Polymorphisms in Mice.

Publications

Association between Y-Maze Acquisition Learning and Major Histocompatibility Complex Class II Polymorphisms in Mice.

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