Ontology highlight
ABSTRACT:
SUBMITTER: Patrinostro X
PROVIDER: S-EPMC6077724 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Patrinostro Xiaobai X Roy Pallabi P Lindsay Angus A Chamberlain Christopher M CM Sundby Lauren J LJ Starker Colby G CG Voytas Daniel F DF Ervasti James M JM Perrin Benjamin J BJ
Proceedings of the National Academy of Sciences of the United States of America 20180716 31
The highly similar cytoplasmic β- and γ-actins differ by only four functionally similar amino acids, yet previous in vitro and in vivo data suggest that they support unique functions due to striking phenotypic differences between <i>Actb</i> and <i>Actg1</i> null mouse and cell models. To determine whether the four amino acid variances were responsible for the functional differences between cytoplasmic actins, we gene edited the endogenous mouse <i>Actb</i> locus to translate γ-actin protein. Th ...[more]