Hypomethylation of GRHL3 gene is associated with the occurrence of neural tube defects.
Ontology highlight
ABSTRACT: Aim
To investigate the relationship between GRHL3 methylation and the etiology of neural tube defects (NTDs).Materials & methods
Analyze data from a genome-wide DNA methylation array. Targeted DNA methylation analysis was performed for 46 cases and 23 controls. At last, grhl3 overexpression and gene depletion experiments were conducted in zebrafish.Results
Five hypomethylated CpGs were discovered in the methylation arrays performed on NTD cases. In a validation study, 15 hypomethylated CpGs were found and the overall methylation levels decreased in brain/spinal cord tissue from NTD cases. The knockdown and overexpression of grhl3 in zebrafish damaged embryonic convergent extension processes.Conclusion
Hypomethylation of GRHL3 in central nervous tissue is associated with NTDs, further supporting the importance of GRHL3 and methylation in proper neural tube closure.
SUBMITTER: Tian T
PROVIDER: S-EPMC6077761 | biostudies-literature | 2018 Jul
REPOSITORIES: biostudies-literature
Publications
Hypomethylation of GRHL3 gene is associated with the occurrence of neural tube defects.
Tian Tian T Wang Linlin L Shen Yan Y Zhang Bo B Finnell Richard H RH Ren Aiguo A
Epigenomics 20180328 7
<h4>Aim</h4>To investigate the relationship between GRHL3 methylation and the etiology of neural tube defects (NTDs).<h4>Materials & methods</h4>Analyze data from a genome-wide DNA methylation array. Targeted DNA methylation analysis was performed for 46 cases and 23 controls. At last, grhl3 overexpression and gene depletion experiments were conducted in zebrafish.<h4>Results</h4>Five hypomethylated CpGs were discovered in the methylation arrays performed on NTD cases. In a validation study, 15 ...[more]
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