Ontology highlight
ABSTRACT:
SUBMITTER: Rodrigues MJ
PROVIDER: S-EPMC6078385 | biostudies-literature | 2017 Mar
REPOSITORIES: biostudies-literature
Nature chemical biology 20170116 3
Substrate channeling has emerged as a common mechanism for enzymatic intermediate transfer. A conspicuous gap in knowledge concerns the use of covalent lysine imines in the transfer of carbonyl-group-containing intermediates, despite their wideuse in enzymatic catalysis. Here we show how imine chemistry operates in the transfer of covalent intermediates in pyridoxal 5'-phosphate biosynthesis by the Arabidopsis thaliana enzyme Pdx1. An initial ribose 5-phosphate lysine imine is converted to the c ...[more]