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Bringing diffuse X-ray scattering into focus.

ABSTRACT: X-ray crystallography is experiencing a renaissance as a method for probing the protein conformational ensemble. The inherent limitations of Bragg analysis, however, which only reveals the mean structure, have given way to a surge in interest in diffuse scattering, which is caused by structure variations. Diffuse scattering is present in all macromolecular crystallography experiments. Recent studies are shedding light on the origins of diffuse scattering in protein crystallography, and provide clues for leveraging diffuse scattering to model protein motions with atomic detail.

SUBMITTER: Wall ME

PROVIDER: S-EPMC6078797 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Bringing diffuse X-ray scattering into focus.

Wall Michael E ME Wolff Alexander M AM Fraser James S JS

Current opinion in structural biology 20180216

X-ray crystallography is experiencing a renaissance as a method for probing the protein conformational ensemble. The inherent limitations of Bragg analysis, however, which only reveals the mean structure, have given way to a surge in interest in diffuse scattering, which is caused by structure variations. Diffuse scattering is present in all macromolecular crystallography experiments. Recent studies are shedding light on the origins of diffuse scattering in protein crystallography, and provide c ...[more]

PMID: 29455056

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