Unknown

Dataset Information

0

Identifying and Validating Tankyrase Binders and Substrates: A Candidate Approach.


ABSTRACT: The poly(ADP-ribose)polymerase (PARP) enzyme tankyrase (TNKS/ARTD5, TNKS2/ARTD6) uses its ankyrin repeat clusters (ARCs) to recognize degenerate peptide motifs in a wide range of proteins, thereby recruiting such proteins and their complexes for scaffolding and/or poly(ADP-ribosyl)ation. Here, we provide guidance for predicting putative tankyrase-binding motifs, based on the previously delineated peptide sequence rules and existing structural information. We present a general method for the expression and purification of tankyrase ARCs from Escherichia coli and outline a fluorescence polarization assay to quantitatively assess direct ARC-TBM peptide interactions. We provide a basic protocol for evaluating binding and poly(ADP-ribosyl)ation of full-length candidate interacting proteins by full-length tankyrase in mammalian cells.

SUBMITTER: Pollock K 

PROVIDER: S-EPMC6082341 | biostudies-literature |

REPOSITORIES: biostudies-literature

Similar Datasets

2017-12-11 | PXD008094 | Pride
| S-EPMC2253340 | biostudies-literature
2009-12-10 | GSE19263 | GEO
| S-EPMC6335875 | biostudies-literature
| S-EPMC8696000 | biostudies-literature
| S-EPMC3947369 | biostudies-literature
| S-EPMC10881709 | biostudies-literature
2009-12-09 | E-GEOD-19263 | biostudies-arrayexpress
| S-EPMC4415586 | biostudies-literature
| S-EPMC5403631 | biostudies-literature