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MiR-221 negatively regulates innate anti-viral response.

ABSTRACT: The innate immune system plays a critical role in the initial antiviral response. However, the timing and duration of these responses must be tightly regulated during infection to ensure appropriate immune cell activation and anti-viral defenses. Here we demonstrate that during antiviral response, a negative regulator miR-221 was also induced in an ELF4-dependent manner. We further show that ELF4 promotes miR-221 expression through direct binding to its promoter. Overexpression and knockdown assay show that miR-221 can negatively regulate IFN? production in time of virus infection. RNA-seq analysis of miR-221 overexpressed cells revealed multiple candidate targets. Taken together, our study identified a novel negative microRNA regulator of innate antiviral response, which is dependent on ELF4.

SUBMITTER: Du H

PROVIDER: S-EPMC6082502 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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MiR-221 negatively regulates innate anti-viral response.

Du Hongqiang H Cui Shuang S Li Yunfei Y Yang Guang G Wang Peiyan P Fikrig Erol E You Fuping F

PloS one 20180808 8

The innate immune system plays a critical role in the initial antiviral response. However, the timing and duration of these responses must be tightly regulated during infection to ensure appropriate immune cell activation and anti-viral defenses. Here we demonstrate that during antiviral response, a negative regulator miR-221 was also induced in an ELF4-dependent manner. We further show that ELF4 promotes miR-221 expression through direct binding to its promoter. Overexpression and knockdown ass ...[more]

PMID: 30089112

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