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Biocatalytic access to diverse prenylflavonoids by combining a regiospecific C-prenyltransferase and a stereospecific chalcone isomerase.

ABSTRACT: Prenylflavonoids are valuable natural products that have diverse biological properties, and are usually generated biologically by multiple metabolic enzymes in nature. In this study, structurally diverse prenylflavonoids were conveniently synthesized by enzymatic catalysis by combining GuILDT, a regiospecific chalcone prenyltransferase, and GuCHI, a stereospecific chalcone isomerase that has promiscuous activity for both chalcones and prenylchalcones as substrates. Our findings provided a new approach for the synthesis of natural/unnatural bioactive prenylflavonoids, including prenylchalcones and optical prenylflavanones with chalcone origins.

SUBMITTER: Li J

PROVIDER: S-EPMC6089845 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Biocatalytic access to diverse prenylflavonoids by combining a regiospecific <i>C</i>-prenyltransferase and a stereospecific chalcone isomerase.

Li Jianhua J Chen Ridao R Wang Ruishan R Liu Xiao X Xie Kebo K Chen Dawei D Dai Jungui J

Acta pharmaceutica Sinica. B 20180305 4

Prenylflavonoids are valuable natural products that have diverse biological properties, and are usually generated biologically by multiple metabolic enzymes in nature. In this study, structurally diverse prenylflavonoids were conveniently synthesized by enzymatic catalysis by combining GuILDT, a regiospecific chalcone prenyltransferase, and GuCHI, a stereospecific chalcone isomerase that has promiscuous activity for both chalcones and prenylchalcones as substrates. Our findings provided a new ap ...[more]

PMID: 30109191

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