Dataset Information

A universal curve of apatite crystallinity for the assessment of bone integrity and preservation.

ABSTRACT: The reliable determination of bioapatite crystallinity is of great practical interest, as a proxy to the physico-chemical and microstructural properties, and ultimately, to the integrity of bone materials. Bioapatite crystallinity is used to diagnose pathologies in modern calcified tissues as well as to assess the preservation state of fossil bones. To date, infrared spectroscopy is one of the most applied techniques for bone characterisation and the derived infrared splitting factor (IRSF) has been widely used to practically assess bioapatite crystallinity. Here we thoroughly discuss and revise the use of the IRSF parameter and its meaning as a crystallinity indicator, based on extensive measurements of fresh and fossil bones, virtually covering the known range of crystallinity degree of bioapatite. A novel way to calculate and use the infrared peak width as a suitable measurement of true apatite crystallinity is proposed, and validated by combined measurement of the same samples through X-ray diffraction. The non-linear correlation between the infrared peak width and the derived ISRF is explained. As shown, the infrared peak width at 604?cm-1 can be effectively used to assess both the average crystallite size and structural carbonate content of bioapatite, thus establishing a universal calibration curve of practical use.

SUBMITTER: Dal Sasso G

PROVIDER: S-EPMC6089980 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

A universal curve of apatite crystallinity for the assessment of bone integrity and preservation.

Dal Sasso Gregorio G Asscher Yotam Y Angelini Ivana I Nodari Luca L Artioli Gilberto G

Scientific reports 20180813 1

The reliable determination of bioapatite crystallinity is of great practical interest, as a proxy to the physico-chemical and microstructural properties, and ultimately, to the integrity of bone materials. Bioapatite crystallinity is used to diagnose pathologies in modern calcified tissues as well as to assess the preservation state of fossil bones. To date, infrared spectroscopy is one of the most applied techniques for bone characterisation and the derived infrared splitting factor (IRSF) has ...[more]

PMID: 30104595

Similar Datasets

Project description:Embryonic stem (ES) cells and germ cells have the potential to give rise to an entire organism. A common requirement is that both must have very robust mechanisms to preserve the integrity of their genomes. This is particularly true since somatic cells have very high mutation frequencies approaching 10-4 in vivo that would lead to unacceptable levels of fetal lethality and congenital defects. Notably, between 70% and 80% of mutational events monitored at a heterozygous endogenous selectable marker were loss of heterozygosity due to mitotic recombination, a mechanism that affects multiple heterozygous loci between the reporter gene and the site of crossing over. This chapter examines three mechanisms by which mouse embryonic stem cells preserve their genomic integrity. The first entails suppression of mutation and recombination between chromosome homologues by two orders of magnitude when compared with isogenic mouse embryo fibroblasts which had a mutation frequency similar to that seen in adult somatic cells. The second renders mouse ES cells hypersensitive to environmental challenge and eliminates damaged cells from the self-renewing population. Mouse ES cells lack a G1 checkpoint so that cells damaged by exogenous insult such as ionizing radiation do not arrest at the G1/S phase checkpoint but progress into the S phase where the damaged DNA is replicated, the damage exacerbated and the cells driven to apoptosis. The third mechanism examines how mouse ES cells repair double strand DNA breaks. Somatic cells predominantly utilize error prone nonhomologous end joining which, from a teleological perspective, would be disadvantageous for ES cells since it would promote accumulation of mutations. When ES cells were tested for the preferred pathway of double strand DNA break repair, they predominantly utilized the high fidelity homology-mediated repair pathway, thereby minimizing the incurrence of mutations during the repair process. When mouse ES cells are induced to differentiate, the predominant repair pathway switches from homology-mediated repair to nonhomologous end joining that is characteristic of somatic cells.

Dataset Information

A universal curve of apatite crystallinity for the assessment of bone integrity and preservation.

Publications

A universal curve of apatite crystallinity for the assessment of bone integrity and preservation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets