Ontology highlight
ABSTRACT:
SUBMITTER: Cao J
PROVIDER: S-EPMC6090611 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Cao Jie J Wei Jianchang J Yang Ping P Zhang Tong T Chen Zhuanpeng Z He Feng F Wei Fang F Chen Huacui H Hu He H Zhong Junbin J Yang Zhi Z Cai Wensong W Li Wanglin W Wang Qiang Q
Molecular cancer 20180813 1
Genome-scale CRISPR-Cas9 Knockout Screening was applied to investigate novel targets in imatinib-resistant gastrointestinal stromal tumor (GIST). 20 genes and 2 miRNAs have been selected by total reads of sgRNA and sgRNA diversity, which has been further validated in imatinib-resistant GIST cells by CCK8 and qPCR analysis. Our study has finally revealed 9 genes (DBP, NR3C1, TCF12, TP53, ZNF12, SOCS6, ZFP36, ACYP1, and DRD1) involved in imatinib-resistant GIST-T1 cells. TP53 and SOCS6 may be the ...[more]