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Cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud.

ABSTRACT: Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools ("cryoem-cloud-tools") have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data processing pipelines. Integrating these tools into the RELION processing pipeline and graphical user interface we determined a 2.2?Å structure of ß-galactosidase in ?55?h on AWS. We implemented a similar strategy to submit Rosetta atomic model building and refinement to AWS. These software tools dramatically reduce the barrier for entry of new users to cloud computing for cryo-EM and are freely available at cryoem-tools.cloud.

SUBMITTER: Cianfrocco MA

PROVIDER: S-EPMC6091888 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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cryoem-cloud-tools: A software platform to deploy and manage cryo-EM jobs in the cloud.

Cianfrocco Michael A MA Lahiri Indrajit I DiMaio Frank F Leschziner Andres E AE

Journal of structural biology 20180601 3

Access to streamlined computational resources remains a significant bottleneck for new users of cryo-electron microscopy (cryo-EM). To address this, we have developed tools that will submit cryo-EM analysis routines and atomic model building jobs directly to Amazon Web Services (AWS) from a local computer or laptop. These new software tools ("cryoem-cloud-tools") have incorporated optimal data movement, security, and cost-saving strategies, giving novice users access to complex cryo-EM data proc ...[more]

PMID: 29864529

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