Ontology highlight
ABSTRACT:
SUBMITTER: Emptage RP
PROVIDER: S-EPMC6092042 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Emptage Ryan P RP Lemmon Mark A MA Ferguson Kathryn M KM Marmorstein Ronen R
Structure (London, England : 1993) 20180628 8
The kinase associated-1 (KA1) domain is found at the C-terminus of multiple Ser/Thr protein kinases from yeast to humans, and has been assigned autoinhibitory, membrane-binding, and substrate-targeting roles. Here, we report the crystal structure of the MARK1 kinase/UBA domain bound to its autoinhibitory KA1 domain, revealing an unexpected interface at the αD helix and contacts with both the N- and C-lobes of the kinase domain. We confirm the binding interface location in kinetic studies of vari ...[more]