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In-solution Y-chromosome capture-enrichment on ancient DNA libraries.

ABSTRACT: BACKGROUND:As most ancient biological samples have low levels of endogenous DNA, it is advantageous to enrich for specific genomic regions prior to sequencing. One approach-in-solution capture-enrichment-retrieves sequences of interest and reduces the fraction of microbial DNA. In this work, we implement a capture-enrichment approach targeting informative regions of the Y chromosome in six human archaeological remains excavated in the Caribbean and dated between 200 and 3000 years BP. We compare the recovery rate of Y-chromosome capture (YCC) alone, whole-genome capture followed by YCC (WGC?+?YCC) versus non-enriched (pre-capture) libraries. RESULTS:The six samples show different levels of initial endogenous content, with very low (

SUBMITTER: Cruz-Davalos DI

PROVIDER: S-EPMC6092841 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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In-solution Y-chromosome capture-enrichment on ancient DNA libraries.

Cruz-Dávalos Diana I DI Nieves-Colón María A MA Sockell Alexandra A Poznik G David GD Schroeder Hannes H Stone Anne C AC Bustamante Carlos D CD Malaspinas Anna-Sapfo AS Ávila-Arcos María C MC

BMC genomics 20180814 1

<h4>Background</h4>As most ancient biological samples have low levels of endogenous DNA, it is advantageous to enrich for specific genomic regions prior to sequencing. One approach-in-solution capture-enrichment-retrieves sequences of interest and reduces the fraction of microbial DNA. In this work, we implement a capture-enrichment approach targeting informative regions of the Y chromosome in six human archaeological remains excavated in the Caribbean and dated between 200 and 3000 years BP. We ...[more]

PMID: 30107783

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