Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin.
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ABSTRACT:
SUBMITTER: Hegemann JD
PROVIDER: S-EPMC6092944 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Publications
Elucidation of the roles of conserved residues in the biosynthesis of the lasso peptide paeninodin.
Hegemann Julian D JD Schwalen Christopher J CJ Mitchell Douglas A DA van der Donk Wilfred A WA
Chemical communications (Cambridge, England) 20180726 65
Substrate binding assays, in vitro proteolytic processing assays, and heterologous lasso peptide production were used to investigate the roles of conserved precursor peptide residues during paeninodin maturation. Specifically, we delineate which residues are important for substrate recognition, proteolysis, and lasso peptide macrocyclization. ...[more]
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