Synaptotagmin oligomerization is essential for calcium control of regulated exocytosis.

ABSTRACT: Regulated exocytosis, which underlies many intercellular signaling events, is a tightly controlled process often triggered by calcium ion(s) (Ca²⁺). Despite considerable insight into the central components involved, namely, the core fusion machinery [soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)] and the principal Ca²⁺ sensor [C2-domain proteins like synaptotagmin (Syt)], the molecular mechanism of Ca²⁺-dependent release has been unclear. Here, we report that the Ca²⁺-sensitive oligomers of Syt1, a conserved structural feature among several C2-domain proteins, play a critical role in orchestrating Ca²⁺-coupled vesicular release. This follows from pHluorin-based imaging of single-vesicle exocytosis

SUBMITTER: Bello OD 

PROVIDER: S-EPMC6094142 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature