Ontology highlight
ABSTRACT:
SUBMITTER: Li-Kroeger D
PROVIDER: S-EPMC6095692 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
Li-Kroeger David D Kanca Oguz O Lee Pei-Tseng PT Cowan Sierra S Lee Michael T MT Jaiswal Manish M Salazar Jose Luis JL He Yuchun Y Zuo Zhongyuan Z Bellen Hugo J HJ
eLife 20180809
We generated two new genetic tools to efficiently tag genes in <i>Drosophila</i>. The first, Double Header (DH) utilizes intronic MiMIC/CRIMIC insertions to generate artificial exons for GFP mediated protein trapping or T2A-GAL4 gene trapping in vivo based on Cre recombinase to avoid embryo injections. DH significantly increases integration efficiency compared to previous strategies and faithfully reports the expression pattern of genes and proteins. The second technique targets genes lacking co ...[more]