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Insight into the molecular mechanism behind PEG-mediated stabilization of biofluid lipases.


ABSTRACT: Bioconjugates established between anionic polyethylene glycol (PEG) based polymers and cationic proteins have proven to be a promising strategy to engineer thermostable biocatalysts. However, the enzyme activity of these bioconjugates is very low and the mechanism of non-covalent PEG-stabilization is yet to be understood. This work presents experimental and molecular dynamics simulation studies, using lipase-polymer surfactant nanoconjugates from mesophile Rhizomucor miehei (RML), performed to evaluate the effect of PEG on enzyme stability and activity. Results demonstrated that the number of hydrogen bonds between the cationized RML and PEG chain correlates with enzyme thermostability. In addition, an increase of both the number of PEG-polymers units and cationization degree of the enzyme leads to a decrease of enzyme activity. Modelling with SAXS data of aqueous solutions of the biofluid lipases agrees with previous hypothesis that these enzymes contain a core constituted of folded protein confined by a shell of surfactants. Together results provide valuable insight into the mechanism of non-covalent PEG mediated protein stabilization relevant for engineering active and thermostable biofluids. Furthermore, the first biofluids RML with activity comparable to their cationized counterpart are presented.

SUBMITTER: Perez B 

PROVIDER: S-EPMC6095910 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Insight into the molecular mechanism behind PEG-mediated stabilization of biofluid lipases.

Pérez Bianca B   Coletta Andrea A   Pedersen Jannik N JN   Petersen Steen V SV   Periole Xavier X   Pedersen Jan Skov JS   Sessions Richard B RB   Guo Zheng Z   Perriman Adam A   Schiøtt Birgit B  

Scientific reports 20180816 1


Bioconjugates established between anionic polyethylene glycol (PEG) based polymers and cationic proteins have proven to be a promising strategy to engineer thermostable biocatalysts. However, the enzyme activity of these bioconjugates is very low and the mechanism of non-covalent PEG-stabilization is yet to be understood. This work presents experimental and molecular dynamics simulation studies, using lipase-polymer surfactant nanoconjugates from mesophile Rhizomucor miehei (RML), performed to e  ...[more]

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