Dataset Information

Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts.

ABSTRACT: Background:Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used in clinical practice, which can have adverse effects on the osteoblast. The objective of this study was to determine the effect of NSAIDs on the osteoblast by analyzing the gene expression of different markers related to osteoblast maturation and function when treated in vitro with different NSAIDs. Methods:Three human osteoblast lines from bone samples of three healthy volunteers were treated with 10 µM acetaminophen, indomethacin, ketoprofen, diclofenac, ibuprofen, ketorolac, naproxen, and piroxicam. The gene expression of different markers (run related transcription factor 2 [RUNX-2], type 1 collagen [COL-I], osterix [OSX], osteocalcin [OSC], bone morphogenetic protein 2 [BMP-2] and 7 [BMP-7], transforming growth factor ?1 [TGF-?1], and TGF? receptors [TGF?R1, TGF?R2; TGFBR3]) were analyzed by real-time PCR at 24 h of treatment. Results:Expression of RUNX-2, COL-I, OSX, was reduced by treatment with all studied NSAIDs, OSC expression was reduced by all NSAIDs except for ketoprofen, naproxen, or piroxicam. Expression of BMP-7 was reduced by all NSAIDs; BMP-2 was reduced by all except for naproxen. In general, NSAID treatment increased the expression of TGF-?1, but not of its receptors (TGF?-R1, TGF?-R2, andTFG?-R3), which was either unchanged or reduced by the treatment. Conclusion:These data confirm that NSAIDs can affect osteoblast physiology, suggesting their possible impact on bone.

SUBMITTER: Melguizo-Rodriguez L

PROVIDER: S-EPMC6097469 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts.

Melguizo-Rodríguez Lucia L Costela-Ruiz Víctor J VJ Manzano-Moreno Francisco J FJ Illescas-Montes Rebeca R Ramos-Torrecillas Javier J García-Martínez Olga O Ruiz Concepción C

PeerJ 20180814

<h4>Background</h4>Nonsteroidal anti-inflammatory drugs (NSAIDs) are frequently used in clinical practice, which can have adverse effects on the osteoblast. The objective of this study was to determine the effect of NSAIDs on the osteoblast by analyzing the gene expression of different markers related to osteoblast maturation and function when treated <i>in vitro</i> with different NSAIDs.<h4>Methods</h4>Three human osteoblast lines from bone samples of three healthy volunteers were treated with ...[more]

PMID: 30128197

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Project description:Nonsteroidal anti-inflammatory drugs (NSAIDs) have been suggested to protect against esophageal adenocarcinoma (EAC). This study examined the effect of NSAIDs on the risk of developing Barrett's esophagus (BE), the precursor lesion to EAC.We conducted a case-control study among eligible patients scheduled for either elective esophagogastroduodenoscopy (EGD) or recruited from primary care clinics to undergo a study EGD. We compared 323 patients with BE (296 nondysplastic and 27 dysplastic) with 2 separate control groups: 1347 patients from the elective EGD group (endoscopy controls) and 502 patients from the primary care group (primary care controls) with no endoscopic or histopathologic BE. Use of aspirin products and 23 nonaspirin NSAIDs was ascertained from detailed, self-reported questionnaires. We calculated odds ratios (ORs) and 95% confidence intervals (CIs) using multivariable logistic regression models.There were no significant differences in self-reported NSAID use between all BE cases and all controls (58.2% vs 54.6%; P = .33); this was seen for aspirin products (43.0% vs 37.4%; P = .08) and nonaspirin NSAIDs (7.7% vs 8.9%; P = .46). These findings persisted in the multivariable model for any NSAIDs (adjusted OR, 0.89; 95% CI, 0.75-1.28), aspirin (adjusted OR, 1.16; 95% CI, 0.90-1.51), and nonaspirin NSAIDs (adjusted OR, 0.88; 95% CI, 0.55-1.39). Use of a combination of aspirin and nonaspirin NSAIDs was reported in 7.4% of cases and 8.3% of controls, and a nonsignificant inverse association with BE was seen (adjusted OR, 0.70; 95% CI, 0.44-1.11). There was no significant association between BE and daily NSAID use (adjusted OR, 1.03; 95% CI, 0.78-1.37). Similar findings were observed for comparisons involving nondysplastic or dysplastic BE cases, and endoscopy or primary care control groups separately or combined.The use of NSAIDs was not associated with a reduced risk of BE. It is likely that the protective mechanism of NSAIDs on EAC occurs subsequent to the development of BE.

Dataset Information

Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts.

Publications

Repercussion of nonsteroidal anti-inflammatory drugs on the gene expression of human osteoblasts.

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