Dataset Information

Bacteria as an Electron Shuttle for Sulfide Oxidation.

ABSTRACT: Biological desulfurization under haloalkaliphilic conditions is a widely applied process, in which haloalkalophilic sulfide-oxidizing bacteria (SOB) oxidize dissolved sulfide with oxygen as the final electron acceptor. We show that these SOB can shuttle electrons from sulfide to an electrode, producing electricity. Reactor solutions from two different biodesulfurization installations were used, containing different SOB communities; 0.2 mM sulfide was added to the reactor solutions with SOB in absence of oxygen, and sulfide was removed from the solution. Subsequently, the reactor solutions with SOB, and the centrifuged reactor solutions without SOB, were transferred to an electrochemical cell, where they were contacted with an anode. Charge recovery was studied at different anode potentials. At an anode potential of +0.1 V versus Ag/AgCl, average current densities of 0.48 and 0.24 A/m2 were measured for the two reactor solutions with SOB. Current was negligible for reactor solutions without SOB. We postulate that these differences in current are related to differences in microbial community composition. Potential mechanisms for charge storage in SOB are proposed. The ability of SOB to shuttle electrons from sulfide to an electrode offers new opportunities for developing a more sustainable desulfurization process.

SUBMITTER: Ter Heijne A

PROVIDER: S-EPMC6097799 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Bacteria as an Electron Shuttle for Sulfide Oxidation.

Ter Heijne Annemiek A de Rink Rieks R Liu Dandan D Klok Johannes B M JBM Buisman Cees J N CJN

Environmental science & technology letters 20180731 8

Biological desulfurization under haloalkaliphilic conditions is a widely applied process, in which haloalkalophilic sulfide-oxidizing bacteria (SOB) oxidize dissolved sulfide with oxygen as the final electron acceptor. We show that these SOB can shuttle electrons from sulfide to an electrode, producing electricity. Reactor solutions from two different biodesulfurization installations were used, containing different SOB communities; 0.2 mM sulfide was added to the reactor solutions with SOB in ab ...[more]

PMID: 30135862

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Project description:Bacteria presumably involved in oxygen- or nitrate-dependent sulfide oxidation in the biofilters of a recirculating marine aquaculture system were identified using a new application of reverse transcription-PCR denaturing gradient gel electrophoresis (DGGE) analysis termed differential-transcription (DT)-DGGE. Biofilter samples were incubated in various concentrations of sulfide or thiosulfate (0 to 5 mM) with either oxygen or nitrate as the sole electron acceptor. Before and after short-term incubations (10 to 20 h), total DNA and RNA were extracted, and a 550-bp fragment of the 16S rRNA genes was PCR amplified either directly or after reverse transcription. DGGE analysis of DNA showed no significant change of the original microbial consortia upon incubation. In contrast, DGGE of cDNA revealed several phylotypes whose relative band intensities markedly increased or decreased in response to certain incubation conditions, indicating enhanced or suppressed rRNA transcription and thus implying metabolic activity under these conditions. Specifically, species of the gammaproteobacterial genus Thiomicrospira and phylotypes related to symbiotic sulfide oxidizers could be linked to oxygen-dependent sulfide oxidation, while members of the Rhodobacteraceae (genera Roseobacter, Rhodobacter, and Rhodobium) were putatively active in anoxic, nitrate-dependent sulfide oxidation. For all these organisms, the physiology of their closest cultured relatives matches their DT-DGGE-inferred function. In addition, higher band intensities following exposure to 5 mM sulfide and nitrate were observed for Thauera-, Hydrogenophaga-, and Dethiosulfovibrio-like phylotypes. For these genera, nitrate-dependent sulfide oxidation has not been documented previously and therefore DT-DGGE might indicate a higher relative tolerance to high sulfide concentrations than that of other community members. We anticipate that DT-DGGE will be of general use in tracing functionally equivalent yet phylogenetically diverse microbial populations in nature.

Dataset Information

Bacteria as an Electron Shuttle for Sulfide Oxidation.

Publications

Bacteria as an Electron Shuttle for Sulfide Oxidation.

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