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Toward Cleavable Metabolic/pH Sensing "Double Agents" Hyperpolarized by NMR Signal Amplification by Reversible Exchange.


ABSTRACT: We show the simultaneous generation of hyperpolarized 13 C-labeled acetate and 15 N-labeled imidazole following spin-relay of hyperpolarization and hydrolysis of the acetyl moiety on 1-13 C-15 N2 -acetylimidazole. Using SABRE-SHEATH (Signal Amplification by Reversible Exchange in SHield Enables Alignment Transfer to Heteronuclei), transfer of spin order occurs from parahydrogen to acetylimidazole 15 N atoms and the acetyl 13 C site (?263-fold enhancement), giving rise to relatively long hyperpolarization lifetimes at 0.3?T (T1 ?52?s and ?149?s for 13 C and 15 N, respectively). Immediately following polarization transfer, the 13 C-labeled acetyl group is hydrolytically cleaved to produce hyperpolarized 13 C-acetate/acetic acid (?140-fold enhancement) and 15 N-imidazole (?180-fold enhancement), the former with a 13 C T1 of ?14?s at 0.3?T. Straightforward synthetic routes, efficient spin-relay of SABRE hyperpolarization, and facile bond cleavage open a door to the cheap and rapid generation of long-lived hyperpolarized states within a wide range of molecular targets, including biologically relevant carboxylic acid derivatives, for metabolic and pH imaging.

SUBMITTER: Kidd BE 

PROVIDER: S-EPMC6097920 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature

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Toward Cleavable Metabolic/pH Sensing "Double Agents" Hyperpolarized by NMR Signal Amplification by Reversible Exchange.

Kidd Bryce E BE   Mashni Jamil A JA   Limbach Miranda N MN   Shi Fan F   Chekmenev Eduard Y EY   Hou Yuqing Y   Goodson Boyd M BM  

Chemistry (Weinheim an der Bergstrasse, Germany) 20180629 42


We show the simultaneous generation of hyperpolarized <sup>13</sup> C-labeled acetate and <sup>15</sup> N-labeled imidazole following spin-relay of hyperpolarization and hydrolysis of the acetyl moiety on 1-<sup>13</sup> C-<sup>15</sup> N<sub>2</sub> -acetylimidazole. Using SABRE-SHEATH (Signal Amplification by Reversible Exchange in SHield Enables Alignment Transfer to Heteronuclei), transfer of spin order occurs from parahydrogen to acetylimidazole <sup>15</sup> N atoms and the acetyl <sup>13<  ...[more]

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