Dataset Information

Co-production of MCR-1 and NDM-5 in Escherichia coli isolated from a colonization case of inpatient.

ABSTRACT: Colistin is increasingly used as an antibiotic of last resort for treating carbapenem-resistant Enterobacteriaceae. Mobile colistin resistance gene mcr-1 has been increasingly reported in Enterobacteriaceae around the world. Of particular concern is the spread of mcr-1 into carbapenemase-producing Enterobacteriaceae, which results in highly drug-resistant strains that are potentially untreatable. Notably, such mcr-1-carrying isolates harboring carbapenemase genes have been reported in animals and patients with infection. Here, we report an Escherichia coli strain carrying co-transferable mcr-1-harboring IncX4 and blaNDM-5-harboring IncX3 plasmids, which was recovered in the context of fecal colonization.

SUBMITTER: Feng S

PROVIDER: S-EPMC6098422 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Co-production of MCR-1 and NDM-5 in Escherichia coli isolated from a colonization case of inpatient.

Feng Siyuan S Shen Cong C Chen Hongtao H Zheng Xiaobin X Xia Yong Y Zhong Lan-Lan LL Huang Xi X Wu Xinwei X Tian Guo-Bao GB

Infection and drug resistance 20180814

Colistin is increasingly used as an antibiotic of last resort for treating carbapenem-resistant Enterobacteriaceae. Mobile colistin resistance gene mcr-1 has been increasingly reported in Enterobacteriaceae around the world. Of particular concern is the spread of mcr-1 into carbapenemase-producing Enterobacteriaceae, which results in highly drug-resistant strains that are potentially untreatable. Notably, such mcr-1-carrying isolates harboring carbapenemase genes have been r ...[more]

PMID: 30147343

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Project description:Background:The concurrence of mcr and carbapenemase genes among Enterobacteriaceae has been a great clinical concern. In our study, we aimed to investigate the prevalence of mcr-positive carbapenem-resistant Enterobacteriaceae (CRE) in fresh vegetables and shed light on the possibility of transmission of mcr-positive CRE via fresh vegetables. Methods:In this study, 712 fresh vegetable samples from 10 provinces in China were collected between May 2017 and Dec 2018 and were screened for mcr and carbapenemase genes. Antibiotic susceptibilities for isolates co-harboring carbapenemase genes and mcr were determined by an agar dilution or a broth microdilution method. Pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analysis were also performed. Transferability of the carbapenemase/mcr-bearing plasmids was determined by conjugation, replicon typing and S1-PFGE-Southern blotting. The sequences of these plasmids were analyzed by using whole-genome sequencing with Illumina Hiseq platform. Results:Two E. coli isolates concomitantly carrying mcr-1 and bla NDM-5/9 from leaf rape and spinach, respectively, were found and both isolates showed multidrug resistance. Notably, mcr-1-positive 690 harboring bla NDM-5 and 701 carrying bla NDM-9 belonged to ST156 and ST2847, respectively, similar to the prevalent MLST types of E. coli co-carrying mcr-1 and bla NDM from avian in our previous study. mcr-1 was on ~33-kb IncX4 plasmid or ~60-kb IncI2 plasmid, while bla NDM-5/9 was on ~46-kb IncX3 plasmid or ~120-kb untypable plasmid. The plasmids were highly similar to those from animals and clinical patients reported in various countries.Conclusion: E. coli isolates concomitantly carrying mcr-1 and bla NDM-5/9 in fresh vegetables may serve as a direct source of pathogens in humans, and such discovery in fresh vegetables emphasizes the importance of prompt surveillance and intervention in limiting the spread of E. coli co-carrying bla NDM and mcr-1. To our knowledge, this is the first report of Enterobacteriaceae co-carrying bla NDM and mcr-1 in fresh vegetables.

Dataset Information

Co-production of MCR-1 and NDM-5 in Escherichia coli isolated from a colonization case of inpatient.

Publications

Co-production of MCR-1 and NDM-5 in <i>Escherichia coli</i> isolated from a colonization case of inpatient.

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