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Genome-wide distribution of linker histone H1.0 is independent of MeCP2.

ABSTRACT: Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional binding of MeCP2 and H1.0 are largely independent.

SUBMITTER: Ito-Ishida A

PROVIDER: S-EPMC6099063 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Genome-wide distribution of linker histone H1.0 is independent of MeCP2.

Ito-Ishida Aya A Yamalanchili Hari Krishna HK Shao Yingyao Y Baker Steven A SA Heckman Laura D LD Lavery Laura A LA Kim Ji-Yoen JY Lombardi Laura M LM Sun Yaling Y Liu Zhandong Z Zoghbi Huda Y HY

Nature neuroscience 20180525 6

Previous studies suggested that MeCP2 competes with linker histone H1, but this hypothesis has never been tested in vivo. Here, we performed chromatin immunoprecipitation followed by sequencing (ChIP-seq) of Flag-tagged-H1.0 in mouse forebrain excitatory neurons. Unexpectedly, Flag-H1.0 and MeCP2 occupied similar genomic regions and the Flag-H1.0 binding was not changed upon MeCP2 depletion. Furthermore, mild overexpression of H1.0 did not alter MeCP2 binding, suggesting that the functional bind ...[more]

PMID: 29802390

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