Ontology highlight
ABSTRACT:
SUBMITTER: Li G
PROVIDER: S-EPMC6099926 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Li Guangyue G Yao Peiyuan P Gong Rui R Li Jinlong J Liu Pi P Lonsdale Richard R Wu Qiaqing Q Lin Jianping J Zhu Dunming D Reetz Manfred T MT
Chemical science 20170331 5
A new directed evolution approach is presented to enhance the activity of an enzyme and to manipulate stereoselectivity by focusing iterative saturation mutagenesis (ISM) simultaneously on residues lining the entrance tunnel and the binding pocket. This combined mutagenesis strategy was applied successfully to the monoamine oxidase from <i>Aspergillus niger</i> (MAO-N) in the reaction of sterically demanding substrates which are of interest in the synthesis of chiral pharmaceuticals based on the ...[more]