Dataset Information

Chemical Profiling and Screening of the Marker Components in the Fruit of Cassia fistula by HPLC and UHPLC/LTQ-Orbitrap MSⁿ with Chemometrics.

ABSTRACT: Cassia fistula L. which is known as “Golden Shower”, is used as an ornamental plant due to its flowers, and fruit parts of this plant have a high medicinal value. There are few reports providing a comprehensive overview of the chemical composition of its fruit or explaining the differences between samples from different sources because of the complexity of its chemical components. The purpose of the present study was to establish a fingerprint evaluation system based on Similarity Analysis (SA), Hierarchical Cluster Analysis (HCA) and Principal Component Analysis (PCA) for the composition identification and quality control of this herb. Twelve samples from Xinjiang and Sichuan provinces in China and India were analyzed by HPLC, and there were fifteen common peaks in the twelve batches. Molecular weight and formula information can be derived from thirty-one peaks by UHPLC/LTQ-Orbitrap MSⁿ, molecular structure information of twenty components was obtained, of which ten compounds were identified by comparison with standard materials. Samples of twelve batches were divided according to their similarity into four groups, which were basically consistent with three different C.fistula fruit-producing areas. Five compounds were finally considered to be chemical markers to determine the quality of this herb. A fingerprints method combined with chemometrics was established to differentiate the origin of the fruit of C. fistula which has the advantages of effectivity and convenience, laying the foundation for the quality evaluation of this herb from different sources.

SUBMITTER: Tan J

PROVIDER: S-EPMC6100387 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Publications

Chemical Profiling and Screening of the Marker Components in the Fruit of Cassia fistula by HPLC and UHPLC/LTQ-Orbitrap MSn with Chemometrics.

Tan Jiawei J Zheng Mengcheng M Duan Susu S Zeng Yanling Y Zhang Ziwei Z Cui Qingyu Q Zhang Jiamei J Hong Tingting T Bai Jie J Du Shouying S

Molecules (Basel, Switzerland) 20180621 7

Cassia fistula L. which is known as “Golden Shower”, is used as an ornamental plant due to its flowers, and fruit parts of this plant have a high medicinal value. There are few reports providing a comprehensive overview of the chemical composition of its fruit or explaining the differences between samples from different sources because of the complexity of its chemical components. The purpose of the present study was to establish a fingerprint evaluation system based on Similarity Analysi ...[more]

PMID: 29933591

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Project description:Pruni Semen, the seed of several unique Prunus plants, is a traditional purgative herbal material. To determine the authentic sources of Pruni Semen, 46 samples from four species were collected and analyzed. Ten compounds including multiflorin A (Mul A), a notable purative compound, were isolated and identified by chemical separation and nuclear magnetic resonance spectroscopy. Seventy-six communal components were identified by ultra-high performance liquid chromatography with linear ion trap-quadrupole Orbitrap mass spectrometry, and acetyl flavonoid glycosides were recognized as characteristic constituents. The flavonoids were distributed in the seed coat and cyanogenic glycosides in the kernel. Based on this, methods for identifying Pruni Semen from different sources were established using chemical fingerprinting, quantitative analysis of the eight principal compounds, hierarchical cluster analysis, principal component analysis, and orthogonal partial least squares discriminant analysis. The results showed that the samples were divided into two categories: one is the small seeds from Prunus humilis (Ph) and Prunus japonica (Pj), and the other is the big seeds from Prunus pedunculata (Pp) and Prunus triloba (Pt). The average content of Mul A was 3.02, 6.93, 0.40, and 0.29 mg/g, while the average content of amygdalin was 18.5, 17.7, 31.5, and 30.9 mg/g in Ph, Pj, Pp, and Pt, respectively. All the above information suggests that small seeds might be superior sources of Pruni Semen. This is the ﬁrst comprehensive report on the identiﬁcation of chemical components in Pruni Semen from different species. Graphical abstract Image 1 Highlights • Chemical constituents of Pruni semen from four Prunus species were compared.• Acetyl flavonoid glycosides were identified as the characteristic components.• Flavonoids were present in the seed coat and cyanogenic glycosides in the kernel.• The content of acetyl flavonoid in small seeds is significant higher than those in big ones.

Dataset Information

Chemical Profiling and Screening of the Marker Components in the Fruit of Cassia fistula by HPLC and UHPLC/LTQ-Orbitrap MSⁿ with Chemometrics.

Publications

Chemical Profiling and Screening of the Marker Components in the Fruit of <i>Cassia fistula</i> by HPLC and UHPLC/LTQ-Orbitrap MS<sup>n</sup> with Chemometrics.

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