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Mass Spectrometry Imaging with Isomeric Resolution Enabled by Ozone-Induced Dissociation.


ABSTRACT: Mass spectrometry imaging (MSI) enables the spatial distributions of molecules possessing different mass-to-charge ratios to be mapped within complex environments revealing regional changes at the molecular level. Even at high mass resolving power, however, these images often reflect the summed distribution of multiple isomeric molecules, each potentially possessing a unique distribution coinciding with distinct biological function(s) and metabolic origin. Herein, this chemical ambiguity is addressed through an innovative combination of ozone-induced dissociation reactions with MSI, enabling the differential imaging of isomeric lipid molecules directly from biological tissues. For the first time, we demonstrate both double bond- and sn-positional isomeric lipids exhibit distinct spatial locations within tissue. This MSI approach enables researchers to unravel local lipid molecular complexity based on both exact elemental composition and isomeric structure directly from tissues.

SUBMITTER: Paine MRL 

PROVIDER: S-EPMC6100449 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Mass Spectrometry Imaging with Isomeric Resolution Enabled by Ozone-Induced Dissociation.

Paine Martin R L MRL   Poad Berwyck L J BLJ   Eijkel Gert B GB   Marshall David L DL   Blanksby Stephen J SJ   Heeren Ron M A RMA   Ellis Shane R SR  

Angewandte Chemie (International ed. in English) 20180619 33


Mass spectrometry imaging (MSI) enables the spatial distributions of molecules possessing different mass-to-charge ratios to be mapped within complex environments revealing regional changes at the molecular level. Even at high mass resolving power, however, these images often reflect the summed distribution of multiple isomeric molecules, each potentially possessing a unique distribution coinciding with distinct biological function(s) and metabolic origin. Herein, this chemical ambiguity is addr  ...[more]

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