Effect of cellular mass on chondrogenic differentiation during embryoid body formation.

ABSTRACT: One approach to cell differentiation is to use the natural capacity of pluripotent stem cells to form three germ layers via embryoid bodies (EB). However, unification of this process during in vitro culture remains challenging and many microenvironmental factors including the number of cells in the culture can influence differentiation patterns. The number of cells serves a crucial role as it determines access to nutrients, the distribution of oxygen concentration and cellular interactions, all of which influence the fate of the differentiated cells. The influence of EBs derived from human pluripotent cells on the chondrogenic potential of such cells is not well understood. For this reason, the present study sought to determine the effect of varying amounts of cells on the properties of EB

SUBMITTER: Lach MS 

PROVIDER: S-EPMC6102628 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature