Dataset Information

Temporal regulation of two cytosolic phosphoglucomutases during stigma development in ornamental kale (Brassica oleracea var. acephala).

ABSTRACT: Phosphoglucomutases (PGM) (5.4.2.2.) belong to the Phosphohexomutases superfamily and are highly specific in catalyzing the interconversion of Glc-1-P to Glc-6-P. In this study, we characterize the expression and activity of two cytosolic PGMs (cPGM2 and cPGM3) stigmas of ornamental kale during flower development. In stigmas, cPGM expression and activity showed a gradual increase during stigma development with the highest activity around the time of anthesis. Blocking of cPGM activity in the stigmas using a known inhibitor, resulted in breakdown of self-incompatibility in immature S3 and S4 stigmas, but had no effect on the fully mature S5 stigmas. It is likely that cPGMs are required for accumulation of factors necessary for SI response in mature stigmas.

SUBMITTER: Li X

PROVIDER: S-EPMC6103284 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Temporal regulation of two cytosolic phosphoglucomutases during stigma development in ornamental kale (Brassica oleracea var. acephala).

Li X X Abhinandan K K Zhang T T Wei D D Li Y Y Lan X X Samuel M A MA

Plant signaling & behavior 20180626 5

Phosphoglucomutases (PGM) (5.4.2.2.) belong to the Phosphohexomutases superfamily and are highly specific in catalyzing the interconversion of Glc-1-P to Glc-6-P. In this study, we characterize the expression and activity of two cytosolic PGMs (cPGM2 and cPGM3) stigmas of ornamental kale during flower development. In stigmas, cPGM expression and activity showed a gradual increase during stigma development with the highest activity around the time of anthesis. Blocking of cPGM activity in the sti ...[more]

PMID: 29944433

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Project description:Ornamental kale (Brassica oleracea L. var. acephala) is a popular decorative plant in late autumn and winter. However, only during low-temperature color-changed periods below rough 15 °C can the plant accumulate anthocyanins and exhibit a diverse array of foliar color patterns. In this study, we probed into the potential mechanism of inner leaf reddening in a red-leaf pure line of ornamental kale by physiological, metabolic, and transcriptomic analyses. Determination of anthocyanin contents in the uncolored new white leaves (S0), the light red leaves (S1) in the reddening period and the red leaves (S2) completing color change, and analysis of anthocyanin metabolites at stage S2, revealed that the coloring of red leaves was mainly attributed to the accumulation of cyanidins. We further used transcriptomic sequencing between the pairwise S0, S1, and S2 stages to identify 21 differentially expressed genes (DEGs) involved in anthocyanin biosynthesis, among which the expression level of 14 DEGs was positively correlated with anthocyanin accumulation, and 6 DEGs were negatively correlated with anthocyanin accumulation. A total of 89 co-expressed genes were screened out, from which three DEGs (BoCHI, Bo4CL3, and BoF3H) were identified as hub genes in co-expression DEGs network. BoDFR and BoCHI were the DEGs with the highest expressions at S2. Moreover, two co-expressed DEGs related to stress response (BoBBX17 and BoCOR47) also exhibited upregulated expressions and positive correlations with anthocyanin accumulation. A deep dive into the underlying regulatory network of anthocyanin accumulation comprising these six upregulated DEGs from S0 to S2 was performed via trend, correlation, and differentially co-expression analysis. This study uncovered the DEGs expression profiles associated with anthocyanin accumulation during ornamental kale inner leaf reddening, which provided a basis for further dissecting the molecular mechanisms of leaf color characteristic change in ornamental kale at low temperatures.

Project description:BACKGROUND:Ornamental cabbage (Brassica oleracea var. acephala) is an attractive landscape plant that remains colorful at low temperatures during winter. Its key feature is its inner leaf coloration, which can include red, pink, lavender, blue, violet and white. Some ornamental cabbages exhibit variation in leaf color pattern linked to leaf developmental stage. However, little is known about the molecular mechanism underlying changes in leaf pigmentation pattern between developmental stages. RESULTS:The transcriptomes of six ornamental cabbage leaf samples were obtained using Illumina sequencing technology. A total of 339.75 million high-quality clean reads were assembled into 46,744 transcripts and 46,744 unigenes. Furthermore, 12,771 genes differentially expressed across the different lines and stages were identified by pairwise comparison. We identified 74 and 13 unigenes as differentially expressed genes related to the anthocyanin biosynthetic pathway and chlorophyll metabolism, respectively. Among them, three unigenes (BoC4H2, BoUGT9, and BoGST21) and six unigenes (BoHEMA1, BoCRD1, BoPORC1, BoPORC2, BoCAO, and BoCLH1) were found as candidates for the genes encoding enzymes in the anthocyanin biosynthetic pathway and chlorophyll metabolism, respectively. In addition, two unigenes (BoRAX3 and BoTRB1) as MYB candidates, two unigenes (BoMUTE1, and BHLH168-like) as bHLH candidates were identified for purple pigmentation in ornamental cabbage. CONCLUSION:Our results indicate that the purple inner leaves of purple ornamental cabbage result from a high level of anthocyanin biosynthesis, a high level of chlorophyll degradation and an extremely low level of chlorophyll biosynthesis, whereas the bicolor (purple/green) outer leaves are due to a moderate level of anthocyanin biosynthesis, a high level of chlorophyll degradation and a very low level of chlorophyll biosynthesis. In white ornamental cabbage, the white inner leaves are due to an extremely low level or absence of anthocyanin biosynthesis, a high level of chlorophyll degradation and a very low level of chlorophyll biosynthesis, whereas the bicolor (white/green) leaves are due to a high level of chlorophyll degradation and a low level of chlorophyll biosynthesis and absence of anthocyanin biosynthesis. These results provide insight into the molecular mechanisms underlying inner and bicolor leaf pigmentation in ornamental cabbage and offer a platform for assessing related ornamental species.

Dataset Information

Temporal regulation of two cytosolic phosphoglucomutases during stigma development in ornamental kale (Brassica oleracea var. acephala).

Publications

Temporal regulation of two cytosolic phosphoglucomutases during stigma development in ornamental kale (Brassica oleracea var. acephala).

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