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Characterization of Brassinazole resistant (BZR) gene family and stress induced expression in Eucalyptus grandis.

ABSTRACT: Brassinosteroids (BRs) are a group of plant hormones which play a pivotal role in modulating cell elongation, stress responses, vascular differentiation and senescence. In response to BRs, BRASSINAZOLE-RESISTANT (BZR) transcription factors (TFs) accumulate in the nucleus, where they modulate thousands of target genes and coordinate many biological processes, especially in regulating defense against biotic and abiotic stresses. In this study, 6 BZR TFs of Eucalyptus grandis (EgrBZR) from a genome-wide survey were characterized by sequence analysis and expression profiling against several abiotic stresses. The results showed that BZR gene family in Eucalyptus was slightly smaller compared to Populus and Arabidopsis, but all phylogenetic groups were represented. Various systematic in silico analysis of these TFs validated the basic properties of BZRs, whereas comparative studies showed a high degree of similarity with recognized BZRs of other plant species. In the organ-specific expression analyses, 4 EgrBZRs were expressed in vascular tissue indicating their possible functions in wood formation. Meanwhile, almost all EgrBZR genes showed differential transcript abundance levels in response to exogenously applied BR, MeJA, and SA, and salt and cold stresses. Besides, protein interaction analysis showed that all EgrBZR genes were associated with BR signaling directly or indirectly. These TFs were proposed as transcriptional activators or repressors of abiotic stress response and growth and development pathways of E. grandis by participating in BR signaling processes. These findings would be helpful in resolving the regulatory mechanism of EgrBZRs in stress resistance conditions but require further functional study of these potential TFs in Eucalyptus.

SUBMITTER: Fan C

PROVIDER: S-EPMC6103948 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Characterization of Brassinazole resistant (BZR) gene family and stress induced expression in Eucalyptus grandis.

Fan Chunjie C Guo Guangsheng G Yan Huifang H Qiu Zhenfei Z Liu Qianyu Q Zeng Bingshan B

Physiology and molecular biology of plants : an international journal of functional plant biology 20180618 5

Brassinosteroids (BRs) are a group of plant hormones which play a pivotal role in modulating cell elongation, stress responses, vascular differentiation and senescence. In response to BRs, BRASSINAZOLE-RESISTANT (BZR) transcription factors (TFs) accumulate in the nucleus, where they modulate thousands of target genes and coordinate many biological processes, especially in regulating defense against biotic and abiotic stresses. In this study, 6 BZR TFs of Eucalyptus grandis (EgrBZR) ...[more]

PMID: 30150857

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Project description:BackgroundThe Xklp2 (TPX2) proteins belong to the microtubule-associated (MAP) family of proteins. All members of the family contain the conserved TPX2 motif, which can interact with microtubules, regulate microtubule dynamics or assist with different microtubule functions, for example, maintenance of cell morphology or regulation of cell growth and development. However, the role of members of the TPX family have not been studied in the model tree species Eucalyptus to date. Here, we report the identification of the members of the TPX2 family in Eucalyptus grandis (Eg) and analyse the expression patterns and functions of these genes.ResultsIn present study, a comprehensive analysis of the plant TPX2 family proteins was performed. Phylogenetic analyses indicated that the genes can be classified into 6 distinct subfamilies. A genome-wide survey identified 12 members of the TPX2 family in the sequenced genome of Eucalyptus grandis. The basic genetic properties of the TPX2 family in Eucalyptus were analysed. Our results suggest that the TPX2 family proteins within different sub-groups are relatively conserved but there are important differences between groups. Quantitative real-time PCR (qRT-PCR) was performed to confirm the expression levels of the genes in different tissues. The results showed that in the whole plant, the levels of EgWDL5 transcript are the highest, followed by those of EgWDL4. Compared with other tissues, the level of the EgMAP20 transcript is the highest in the root. Over-expression of EgMAP20 in Arabidopsis resulted in organ twisting. The cotyledon petioles showed left-handed twisting while the hypocotyl epidermal cells produced right-handed helical twisting. Finally, EgMAP20, EgWDL3 and EgWDL3L were all able to decorate microtubules.ConclusionsPlant TPX2 family proteins were systematically analysed using bioinformatics methods. There are 12 TPX2 family proteins in Eucalyptus. We have performed an initial characterization of the functions of several members of the TPX2 family. We found that the gene products are localized to the microtubule cytoskeleton. Our results lay the foundation for future efforts to reveal the biological significance of TPX2 family proteins in Eucalyptus.

Dataset Information

Characterization of Brassinazole resistant (BZR) gene family and stress induced expression in Eucalyptus grandis.

Publications

Characterization of <i>Brassinazole resistant</i> (<i>BZR</i>) gene family and stress induced expression in <i>Eucalyptus grandis</i>.

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