Dataset Information

Dataset on the beneficiation of a Nigerian bentonite clay mineral for drilling mud formulation.

ABSTRACT: This paper presents dataset on the beneficiation of a Nigerian clay mineral for drilling mud application. The experimental design applied used a Response Surface Design (RSM), which involved 24 (2-Level, 4-Factors) to generate statistical models, and analyze the dataset. The independent variables were (Bentonite; X1), (Polymer; X2), (Sodium Carbonate, X3) and (Aging Time; X4). The rheological properties of interest, which forms the response variables, were selected based on the API specification 13-A for drilling grade bentonite. The outcomes show that the second-order statistical models derived from responses fitted well with the experimental results. Predictive models obtained from the statistical characterization of the beneficiation process would allow for the design and cost-effective planning of the procedure. The beneficiation of the clay using sodium carbonate and Kelzan® XCD polymer ensued in an improvement in the rheological properties of the formulated drilling mud. These properties were comparable with the API specification 13-A for drilling fluid materials.

SUBMITTER: Afolabi RO

PROVIDER: S-EPMC6104560 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Dataset on the beneficiation of a Nigerian bentonite clay mineral for drilling mud formulation.

Afolabi Richard O RO Ogunkunle Temitope F TF Olabode Oluwasanmi A OA Yusuf Esther O EO

Data in brief 20180803

This paper presents dataset on the beneficiation of a Nigerian clay mineral for drilling mud application. The experimental design applied used a Response Surface Design (RSM), which involved 24 (2-Level, 4-Factors) to generate statistical models, and analyze the dataset. The independent variables were (Bentonite; X1), (Polymer; X2), (Sodium Carbonate, X3) and (Aging Time; X4). The rheological properties of interes ...[more]

PMID: 30140719

Similar Datasets

Project description:Bentonite clay is an integral component of the engineered barrier system of deep geological repositories (DGRs) that are planned for the long-term storage of high-level radioactive waste. Although nucleic acid extraction and analysis can provide powerful qualitative and quantitative data reflecting the presence, abundance, and functional potential of microorganisms within DGR materials, extraction of microbial DNA from bentonite clay is challenging due to the low biomass and adsorption of nucleic acids to the charged clay matrix. In this study, we used quantitative PCR, gel fingerprinting, and high-throughput sequencing of 16S rRNA gene amplicons to assess DNA extraction efficiency from natural MX-80 bentonite and the same material "spiked" with Escherichia coli genomic DNA. Extraction protocols were tested without additives and with casein and phosphate as blocking agents. Although we demonstrate improved DNA recovery by blocking agents at relatively high DNA spiking concentrations, at relatively low spiking concentrations, we detected a high proportion of contaminant nucleic acids from blocking agents that masked sample-specific microbial profile data. Because bacterial genomic DNA associated with casein preparations was insufficiently removed by UV treatment, casein is not recommended as an additive for DNA extractions from low-biomass samples. Instead, we recommend a kit-based extraction protocol for bentonite clay without additional blocking agents, as tested here and validated with multiple MX-80 bentonite samples, ensuring relatively high DNA recoveries with minimal contamination.IMPORTANCE Extraction of microbial DNA from MX-80 bentonite is challenging due to low biomass and adsorption of nucleic acid molecules to the charged clay matrix. Blocking agents improve DNA recovery, but their impact on microbial community profiles from low-biomass samples has not been characterized well. In this study, we evaluated the effect of casein and phosphate as blocking agents for quantitative recovery of nucleic acids from MX-80 bentonite. Our data justify a simplified framework for analyzing microbial community DNA associated with swelling MX-80 bentonite samples within the context of a deep geological repository for used nuclear fuel. This study is among the first to demonstrate successful extraction of DNA from Wyoming MX-80 bentonite.

Dataset Information

Dataset on the beneficiation of a Nigerian bentonite clay mineral for drilling mud formulation.

Publications

Dataset on the beneficiation of a Nigerian bentonite clay mineral for drilling mud formulation.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets