Dataset Information

Overexpression of a High-Affinity Nitrate Transporter OsNRT2.1 Increases Yield and Manganese Accumulation in Rice Under Alternating Wet and Dry Condition.

ABSTRACT: Nitrate and manganese (Mn) are necessary elements for the growth and development of rice in paddy soil. Under physiological conditions, we previously reported that the uptake of Mn in roots can be improved by the addition of external nitrate but not ammonium. To investigate the mechanism(s) of this phenotype, we produced plant lines overexpressing OsNRT2.1 and assessed Mn uptake under alternating wet and dry (AWD) and waterlogged (WL) conditions. Under AWD condition, we observed a 31% reduction in grain yields of wild type (WT) plants compared to WL condition. Interestingly, the overexpression of OsNRT2.1 could recover this loss, as OsNRT2.1 transgenic lines displayed higher grain yields than WT plants. We also observed 60% higher grain Mn in the transgenic lines in AWD condition and approximately 30% higher Mn in the grain of transgenic lines in WL condition. We further found that the overexpression of OsNRT2.1 did not alter Mg and Fe in the seeds in either growth condition. The reasons for the increased Mn content in OsNRT2.1 transgenic seeds in AWD condition could be explained by the elevated expression of OsNRAMP family genes including OsNRAMP3, OsNRAMP5, and OsNRAMP6 in node I, the panicle-neck, and the flag leaves. The mechanism(s) underpinning the upregulation of these genes requires further investigation. Taken together, our results provide a new function of OsNRT2.1 in improving rice yields and grain Mn accumulation during water-saving cultivation patterns. This represents a new strategy for maintaining yield and improving food quality in a sustainable agricultural system.

SUBMITTER: Luo B

PROVIDER: S-EPMC6104626 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

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Overexpression of a High-Affinity Nitrate Transporter <i>OsNRT2.1</i> Increases Yield and Manganese Accumulation in Rice Under Alternating Wet and Dry Condition.

Luo Bingbing B Chen Jingguang J Zhu Longlong L Liu Shuhua S Li Bin B Lu Hong H Ye Guoyou G Xu Guohua G Fan Xiaorong X

Frontiers in plant science 20180815

Nitrate and manganese (Mn) are necessary elements for the growth and development of rice in paddy soil. Under physiological conditions, we previously reported that the uptake of Mn in roots can be improved by the addition of external nitrate but not ammonium. To investigate the mechanism(s) of this phenotype, we produced plant lines overexpressing <i>OsNRT2.1</i> and assessed Mn uptake under alternating wet and dry (AWD) and waterlogged (WL) conditions. Under AWD condition, we observed a 31% red ...[more]

PMID: 30158947

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Project description:BACKGROUND:Rice tiller number is one of the most important factors that determine grain yield, while nitrogen is essential for the crop growth and development, especially for tiller formation. Genes involved in nitrogen use efficiency processes have been identified in the previous studies, however, only a small number of these genes have been found to improve grain yield by promoting tillering. RESULTS:We constructed over-expression (OX) lines and RNA-interference (Ri) lines, and selected a mutant of OsNPF7.2, a low-affinity nitrate transporter. Our analyses showed that rice tiller number and grain yield were significantly increased in OX lines, whereas Ri lines and mutant osnpf7.2 had fewer tiller number and lower grain yield. Under different nitrate concentrations, tiller buds grew faster in OX lines than in WT, but they grew slower in Ri lines and mutant osnpf7.2. These results indicated that altered expression of OsNPF7.2 plays a significant role in the control of tiller bud growth and regulation of tillering. Elevated expression of OsNPF7.2 also improved root length, root number, fresh weight, and dry weight. However, reduced expression of OsNPF7.2 had the opposite result on these characters. OsNPF7.2 OX lines showed more significantly enhanced influx of nitrate and had a higher nitrate concentration than WT. The levels of gene transcripts related to cytokinin pathway and cell cycle in tiller bud, and cytokinins concentration in tiller basal portion were higher in OX lines than that in WT, suggesting that altered expression of OsNPF7.2 controlled tiller bud growth and root development by regulating cytokinins content and cell cycle in plant cells. Altered expression of OsNPF7.2 also was responsible for the change in expression of the genes involved in strigolactone pathway, such as D27, D17, D10, Os900, Os1400, D14, D3, and OsFC1. CONCLUSION:Our results suggested that OsNPF7.2 is a positive regulator of nitrate influx and concentration, and that it also regulates cell division in tiller bud and alters expression of genes involved in cytokinin and strigolactone pathways, resulting in the control over rice tiller number. Since elevated expression of OsNPF7.2 is capable of improving rice grain yield, this gene might be applied to high-yield rice breeding.

Project description:Canopy leaves are sinks of dry and wet nitrogen (N) deposition, most studies have not considered the response of canopy leaves to the alternating processes of dry and wet N deposition. We manipulated a close top chamber experiment to observe the effects of simulated N deposition with the same total deposition flux but different dry to wet ratios on leaf structure and physiology by spraying NH4Cl solution or supplying gaseous NH3 over the canopy of seedlings of three species (Betula platyphylla, Fraxinus mandshurica, Pinus koraiensis) placed in the chamber. After 32 days of N deposition and relative to the control, the leaf morphology and mesophyll tissue structure of the three species had no significant changes under all N deposition treatments. With the increase in the ratio of dry to wet N deposition, the N concentration, N metabolizing enzyme activity and soluble protein concentration in leaves of all three species increased continuously, but for the leaf light-saturated net photosynthesis rate, B. platyphylla showed a continuous increase, F. mandshurica showed a continuous decrease, and P. koraiensis showed no significant change. We found that F. mandshurica was the only species whose foliar chlorophyll and potassium concentration decreased with the increase in the ratio of dry to wet N deposition and its leaf light-saturated net photosynthesis rate was positively correlated with foliar chlorophyll and potassium concentration, respectively. Our results indicate that dry deposition is relatively more important on leaf physiological functions in alternating deposition. B. platyphylla and P. koraiensis may better acclimate to canopy NH3/NH4 + deposition than F. mandshurica. Most importantly, the results indicate that a single simulated dry and wet deposition would overestimate and underestimate the response of leaf function to atmospheric N deposition, respectively. Alternating processes of dry and wet deposition should be considered for more realistic assessments of the effects of atmospheric N deposition in forests.

Dataset Information

Overexpression of a High-Affinity Nitrate Transporter OsNRT2.1 Increases Yield and Manganese Accumulation in Rice Under Alternating Wet and Dry Condition.

Publications

Overexpression of a High-Affinity Nitrate Transporter <i>OsNRT2.1</i> Increases Yield and Manganese Accumulation in Rice Under Alternating Wet and Dry Condition.

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