Recommendations To Address the Difficulties Encountered When Determining Linezolid Resistance from Whole-Genome Sequencing Data.
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ABSTRACT:
SUBMITTER: Beukers AG
PROVIDER: S-EPMC6105777 | biostudies-literature | 2018 Aug
REPOSITORIES: biostudies-literature
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Recommendations To Address the Difficulties Encountered When Determining Linezolid Resistance from Whole-Genome Sequencing Data.
Beukers Alicia G AG Hasman Henrik H Hegstad Kristin K van Hal Sebastiaan J SJ
Antimicrobial agents and chemotherapy 20180727 8
Mutations associated with linezolid resistance within the V domain of 23S rRNA are annotated using an <i>Escherichia coli</i> numbering system. The 23S rRNA gene varies in length, nucleotide sequence, and copy number among bacterial species. Consequently, this numbering system is not intuitive and can lead to confusion when mutation sites are being located using whole-genome sequencing data. Using the mutation G2576T as an example, we demonstrate the difficulties associated with using the <i>E. ...[more]
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