Standardisation of basal medium for reproducible culture of human annulus fibrosus and nucleus pulposus cells.

ABSTRACT: BACKGROUND:The lifetime prevalence of degenerative disc disease is dramatically high. Numerous investigations on disc degeneration have been performed on cells from annulus fibrosus (AF) and nucleus pulposus (NP) of the intervertebral disc (IVD) in cell culture experiments utilising a broad variety of basal culture media. Although the basal media differ in nutrient formulation, it is not known whether the choice of the basal media itself has an impact on the cell's behaviour in vitro. In this study, we evaluated the most common media used for monolayer expansion of AF and NP cells to set standards for disc cell culture. METHODS:Human AF and NP cells were isolated from cervical discs. Cells were expanded in monolayer until passage P2 using six different common culture media containing alpha

SUBMITTER: Schubert AK 

PROVIDER: S-EPMC6106880 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature