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CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts.

ABSTRACT: Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and robust activation of capping to dissect the mechanism of co-transcriptional capping. Taken together, our findings identify a CTD-independent, but Pol II-mediated, mechanism that functions in parallel with CTD-dependent processes to ensure optimal capping, and they support a "tethering" model for the mechanism of activation.

SUBMITTER: Noe Gonzalez M

PROVIDER: S-EPMC6107522 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts.

Noe Gonzalez Melvin M Sato Shigeo S Tomomori-Sato Chieri C Conaway Joan W JW Conaway Ronald C RC

Nature communications 20180823 1

Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and r ...[more]

PMID: 30139934

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