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Screening, purification and characterization of lipase from Burkholderia pyrrocinia B1213.

ABSTRACT: A lipase producing strain B1213 isolated from soil was identified as Burkholderia pyrrocinia based on 16S rRNA gene and recA sequeence analysis, making this the first report on the presence of a lipase from B. pyrrocinia. Under an aqueous two-phase purification strategy, which included (ATPE)-ion-exchange chromatography (IEC)-gel and filtration chromatography (GFC), the specific activity of the 35-kDa lipase was determined to be 875.7 U/mg protein. The optimum pH and temperature of this lipase was pH 8.0 and 50 °C, respectively. The lipase retained?>?85% activity in isopropanol and acetone at 30 °C for 10 min but the activity was reduced to 10.6% in n-hexane. Mg²⁺, Al³⁺, Mn²⁺, and Fe³⁺ enhanced lipase activity at both 1 mM and 5 mM concentrations. p-NPP, a long-chain acyl group 4-NP ester, appeared to be a good substrate candidate.

SUBMITTER: Li J

PROVIDER: S-EPMC6109439 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Screening, purification and characterization of lipase from Burkholderia pyrrocinia B1213.

Li Jinlong J Shen Weijia W Fan Guangsen G Li Xiuting X

3 Biotech 20180825 9

A lipase producing strain B1213 isolated from soil was identified as Burkholderia pyrrocinia based on 16S rRNA gene and recA sequeence analysis, making this the first report on the presence of a lipase from B. pyrrocinia. Under an aqueous two-phase purification strategy, which included (ATPE)-ion-exchange chromatography (IEC)-gel and filtration chromatography (GFC), the specific activity of the 35-kDa lipase was determined to be 875.7 U/mg protein. The optimum pH and temperature of ...[more]

PMID: 30175024

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