Dataset Information

Tricarboxylates Induce Defense Priming Against Bacteria in Arabidopsis thaliana.

ABSTRACT: Exposure of plants to biotic stress results in an effective induction of numerous defense mechanisms that involve a vast redistribution within both primary and secondary metabolisms. For instance, an alteration of tricarboxylic acid (TCA) levels can accompany the increase of plant resistance stimulated by various synthetic and natural inducers. Moreover, components of the TCA flux may play a role during the set-up of plant defenses. In this study, we show that citrate and fumarate, two major components of the TCA cycle, are able to induce priming in Arabidopsis against the bacterial pathogen Pseudomonas syringae pv. tomato DC3000. Both citrate and fumarate show no direct antimicrobial effect and therefore enhanced bacterial resistance found in planta is solely based on the induction of the plant defense system. During the priming phase, both TCA intermediates did not induce any changes in transcript abundances of a set of defense genes, and in phytohormones and camalexin levels. However, at early time points of bacterial challenge, citrate induced a stronger salicylic acid and camalexin accumulation followed later by a boost of the jasmonic acid pathway. On the other hand, adaptations of hormonal pathways in fumarate-treated plants were more complex. While jasmonic acid was not induced, mutants impaired in jasmonic acid perception failed to mount a proper priming response induced by fumarate. Our results suggest that changes in carboxylic acid abundances can enhance Arabidopsis defense through complex signaling pathways. This highlights a promising feature of TCAs as novel defense priming agents and calls for further exploration in other pathosystems and stress situations.

SUBMITTER: Balmer A

PROVIDER: S-EPMC6110165 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Tricarboxylates Induce Defense Priming Against Bacteria in <i>Arabidopsis thaliana</i>.

Balmer Andrea A Pastor Victoria V Glauser Gaetan G Mauch-Mani Brigitte B

Frontiers in plant science 20180820

Exposure of plants to biotic stress results in an effective induction of numerous defense mechanisms that involve a vast redistribution within both primary and secondary metabolisms. For instance, an alteration of tricarboxylic acid (TCA) levels can accompany the increase of plant resistance stimulated by various synthetic and natural inducers. Moreover, components of the TCA flux may play a role during the set-up of plant defenses. In this study, we show that citrate and fumarate, two major com ...[more]

PMID: 30177948

Similar Datasets

Project description:Plants must continuously react to the ever-fluctuating nature of their environment. Repeated exposure to stressful conditions can lead to priming, whereby prior encounters heighten a plant's ability to respond to future events. A clear example of priming is provided by the model plant Arabidopsis thaliana (Arabidopsis), in which photosynthetic and photoprotective responses are enhanced following recurring light stress. While there are various post-translational mechanisms underpinning photoprotection, an unresolved question is the relative importance of transcriptional changes toward stress priming and, consequently, the potential contribution from DNA methylation - a heritable chemical modification of DNA capable of influencing gene expression. Here, we systematically investigate the potential molecular underpinnings of physiological priming against recurring excess-light (EL), specifically DNA methylation and transcriptional regulation: the latter having not been examined with respect to EL priming. The capacity for physiological priming of photosynthetic and photoprotective parameters following a recurring EL treatment was not impaired in Arabidopsis mutants with perturbed establishment, maintenance, or removal of DNA methylation. Importantly, no differences in development or basal photoprotective capacity were identified in the mutants that may confound the above result. Little evidence for a causal transcriptional component of physiological priming was identified; in fact, most alterations in primed plants presented as a transcriptional 'dampening' in response to an additional EL exposure, likely a consequence of physiological priming. However, a set of transcripts uniquely regulated in primed plants provide preliminary evidence for a novel transcriptional component of recurring EL priming, independent of physiological changes. Thus, we propose that physiological priming of recurring EL in Arabidopsis occurs independently of DNA methylation; and that the majority of the associated transcriptional alterations are a consequence, not cause, of this physiological priming.

Dataset Information

Tricarboxylates Induce Defense Priming Against Bacteria in Arabidopsis thaliana.

Publications

Tricarboxylates Induce Defense Priming Against Bacteria in <i>Arabidopsis thaliana</i>.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets