Evaluation of six regions for their potential as DNA barcodes in epiphyllous liverworts from Thailand.

ABSTRACT:

Premise of the study

Studies on the diversity of epiphyllous bryophytes have been limited because of minute and incomplete specimens and a lack of taxonomic expertise. The recent development of the DNA barcoding approach has allowed taxon identification and species discovery of many obscure groups of organisms.

Methods

With DNA extractions from 99 samples of 16 species, we compared the efficiencies of six DNA markers (rbcL, matK, trnL-F, psbA, ITS1, and ITS2) in their ability to amplify, using a standard set of primers, as well as their discriminatory power, using distance-based and tree-based approaches with nucleotide data.

Results

The amplification success was relatively high (70-90%) with all of the markers, except for matK, whic

SUBMITTER: Yodphaka S 

PROVIDER: S-EPMC6110246 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature