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Evaluation of six regions for their potential as DNA barcodes in epiphyllous liverworts from Thailand.


ABSTRACT: Premise of the Study:Studies on the diversity of epiphyllous bryophytes have been limited because of minute and incomplete specimens and a lack of taxonomic expertise. The recent development of the DNA barcoding approach has allowed taxon identification and species discovery of many obscure groups of organisms. Methods:With DNA extractions from 99 samples of 16 species, we compared the efficiencies of six DNA markers (rbcL, matK, trnL-F, psbA, ITS1, and ITS2) in their ability to amplify, using a standard set of primers, as well as their discriminatory power, using distance-based and tree-based approaches with nucleotide data. Results:The amplification success was relatively high (70-90%) with all of the markers, except for matK, which yielded no success. The barcoding gap, as calculated from the difference between inter- and intraspecific genetic distances, was the highest in ITS2, whereas the highest numbers of monophyletic groups were found with ITS2 and rbcL. Discussion:rbcL should be used as a main barcoding marker with the addition of ITS2 for epiphyllous species. The development of DNA barcoding as a tool for quantifying species diversity will provide a rapid and reliable identification tool for epiphyllous bryophytes.

SUBMITTER: Yodphaka S 

PROVIDER: S-EPMC6110246 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Evaluation of six regions for their potential as DNA barcodes in epiphyllous liverworts from Thailand.

Yodphaka Sorrasak S   Boonpragob Kansri K   Lumbsch H Thorsten HT   Kraichak Ekaphan E  

Applications in plant sciences 20180820 8


<h4>Premise of the study</h4>Studies on the diversity of epiphyllous bryophytes have been limited because of minute and incomplete specimens and a lack of taxonomic expertise. The recent development of the DNA barcoding approach has allowed taxon identification and species discovery of many obscure groups of organisms.<h4>Methods</h4>With DNA extractions from 99 samples of 16 species, we compared the efficiencies of six DNA markers (<i>rbcL</i>, <i>matK</i>, <i>trnL-F</i>, <i>psbA</i>, ITS1, and  ...[more]

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