Impact of euthanasia, dissection and postmortem delay on metabolic profile in mouse retina and RPE/choroid.

ABSTRACT: Metabolomics studies in the retina and retinal pigment epithelium (RPE) in animal models or postmortem donors are essential to understanding the retinal metabolism and to revealing the underlying mechanisms of retinal degenerative diseases. We have studied how different methods of euthanasia (CO₂ or cervical dislocation) different isolation procedures and postmortem delay affect metabolites in mouse retina and RPE/choroid using LC MS/MS and GC MS. Compared with cervical dislocation, CO₂ exposure for 5 min dramatically degrades ATP and GTP into purine metabolites in the retina while raising intermediates in glucose metabolism and amino acids in the RPE/choroid. Isolation in cold buffer containing glucose has the least change in metabolites. Postmortem delay time-depend

SUBMITTER: Zhu S 

PROVIDER: S-EPMC6110973 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature