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Impact of euthanasia, dissection and postmortem delay on metabolic profile in mouse retina and RPE/choroid.

ABSTRACT: Metabolomics studies in the retina and retinal pigment epithelium (RPE) in animal models or postmortem donors are essential to understanding the retinal metabolism and to revealing the underlying mechanisms of retinal degenerative diseases. We have studied how different methods of euthanasia (CO2 or cervical dislocation) different isolation procedures and postmortem delay affect metabolites in mouse retina and RPE/choroid using LC MS/MS and GC MS. Compared with cervical dislocation, CO2 exposure for 5?min dramatically degrades ATP and GTP into purine metabolites in the retina while raising intermediates in glucose metabolism and amino acids in the RPE/choroid. Isolation in cold buffer containing glucose has the least change in metabolites. Postmortem delay time-dependently and differentially impacts metabolites in the retina and RPE/choroid. In the postmortem retina, 18% of metabolites were changed at 0.5?h (h), 41% at 4?h and 51% at 8?h. However, only 6% of metabolites were changed in the postmortem RPE/choroid and it steadily increased to 20% at 8?h. Notably, both postmortem retina and RPE/choroid tissue showed increased purine metabolites. Storage of eyes in cold nutrient-rich medium substantially blocked the postmortem change in the retina and RPE/choroid. In conclusion, our study provides optimized methods to prepare fresh or postmortem retina and RPE/choroid tissue for metabolomics studies.

SUBMITTER: Zhu S

PROVIDER: S-EPMC6110973 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Impact of euthanasia, dissection and postmortem delay on metabolic profile in mouse retina and RPE/choroid.

Zhu Siyan S Yam Michelle M Wang Yekai Y Linton Jonathan D JD Grenell Allison A Hurley James B JB Du Jianhai J

Experimental eye research 20180601

Metabolomics studies in the retina and retinal pigment epithelium (RPE) in animal models or postmortem donors are essential to understanding the retinal metabolism and to revealing the underlying mechanisms of retinal degenerative diseases. We have studied how different methods of euthanasia (CO<sub>2</sub> or cervical dislocation) different isolation procedures and postmortem delay affect metabolites in mouse retina and RPE/choroid using LC MS/MS and GC MS. Compared with cervical dislocation, C ...[more]

PMID: 29864440

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Impact of euthanasia, dissection and postmortem delay on metabolic profile in mouse retina and RPE/choroid.

Publications

Impact of euthanasia, dissection and postmortem delay on metabolic profile in mouse retina and RPE/choroid.

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