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Sequence and phylogenetic analysis of canine parvovirus-2 isolates in dogs revealed circulation of three subtypes in Nigeria.

ABSTRACT: Canine parvovirus-2 (CPV-2) is the most common viral cause of gastroenteritis in puppies in Nigeria and worldwide. After its emergence in 1978, the wild-type CPV-2 was rapidly replaced by three antigenic subtypes CPV-2a, CPV-2b and CPV-2c. Subtype CPV-2a has been reported in Nigeria based on limited number of samples. As such, this study was carried out with 56 faecal samples and four CPV-2 vaccines using polymerase chain reaction and sequencing to have a better idea of CPV-2 subtypes circulating in dogs in Nigeria. 54 (96.4%) out of the 56 samples were positive for CPV-2 subtypes. CPV-2a is the predominant subtype followed by CPV-2b and CPV-2c. Also, co-existence of subtypes CPV-2a and CPV-2b; and CPV-2a and CPV-2c were found in some dogs. Sequence analysis revealed the occurrence of three mutations D413 N, N426D/E, and T440A. To our knowledge, this is the first report of the three subtypes of CPV-2 in Nigeria.

SUBMITTER: Fagbohun OA

PROVIDER: S-EPMC6111946 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

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Sequence and phylogenetic analysis of canine parvovirus-2 isolates in dogs revealed circulation of three subtypes in Nigeria.

Fagbohun O A OA Omobowale T O TO

Virusdisease 20180728 3

Canine parvovirus-2 (CPV-2) is the most common viral cause of gastroenteritis in puppies in Nigeria and worldwide. After its emergence in 1978, the wild-type CPV-2 was rapidly replaced by three antigenic subtypes CPV-2a, CPV-2b and CPV-2c. Subtype CPV-2a has been reported in Nigeria based on limited number of samples. As such, this study was carried out with 56 faecal samples and four CPV-2 vaccines using polymerase chain reaction and sequencing to have a better idea of CPV-2 subtypes circulatin ...[more]

PMID: 30159381

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Project description:AimThis study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018.Materials and methodsA total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis.ResultsOut of 50 fecal samples, 20 samples were positive (40%) by Rapid CPV/canine coronavirus Ag Test Kit. These positive samples were cultured successfully on MDCK cells. Nine randomly chosen samples out of 30 apparently negative samples were amplified using PCR with primers Hfor and Hrev to yield a typical 630 bp fragment. Then, six randomly chosen samples out of nine were amplified using PCR with primers Pbs and Pbas to yield a typical 427 bp fragment. Sequencing, BLAST analysis and assembly of the two fragments (630 bp and 427 bp) to produce 912 bp fragments, in the six samples, revealed two serotypes CPV-2b and CPV-2c. The obtained strains were submitted to GenBank and given accession numbers MK642272, MK642273, MK642274, MK642275, MK642276, and MK642277. Phylogenetic analysis of the Egyptian strains serotype 2b illustrated that they were closely related to Thailand strains (accession numbers KP715709, KP715694, KP715701, and KP715700); while Egyptian strains serotype 2c was closely related to Thailand strains (accession numbers MH711894 and MH711902), Taiwanese strain (KU244254), Chinese strain (MF467242), and Vietnamese strain (accession number LC216910).ConclusionThe current research recommends further epidemiological studies to assess the extent of the occurrence of different serotypes of CPV in Egypt and the efficiency of imported and locally produced vaccines in protection against CPV infection.

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Sequence and phylogenetic analysis of canine parvovirus-2 isolates in dogs revealed circulation of three subtypes in Nigeria.

Publications

Sequence and phylogenetic analysis of canine parvovirus-2 isolates in dogs revealed circulation of three subtypes in Nigeria.

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