Dataset Information

Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples.

ABSTRACT: An increasing production of natural rubber (NR) products has led to major challenges in waste management. In this study, the degradation of rubber latex gloves in a mineral salt medium (MSM) using a bacterial consortium, a mixed culture of the selected bacteria and a pure culture were studied. The highest 18% weight loss of the rubber gloves were detected after incubated with the mixed culture. The increased viable cell counts over incubation time indicated that cells used rubber gloves as sole carbon source leading to the degradation of the polymer. The growth behavior of NR-degrading bacteria on the latex gloves surface was investigated using the scanning electron microscope (SEM). The occurrence of the aldehyde groups in the degradation products was observed by Fourier Transform Infrared Spectroscopy analysis. Rhodococcus pyridinivorans strain F5 gave the highest weight loss of rubber gloves among the isolated strain and posses latex clearing protein encoded by lcp gene. The mixed culture of the selected strains showed the potential in degrading rubber within 30 days and is considered to be used efficiently for rubber product degradation. This is the first report to demonstrate a strong ability to degrade rubber by Rhodococcus pyridinivorans.

SUBMITTER: Nawong C

PROVIDER: S-EPMC6112053 | biostudies-literature | 2018 Jul - Sep

REPOSITORIES: biostudies-literature

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Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples.

Nawong Chairat C Umsakul Kamontam K Sermwittayawong Natthawan N

Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] 20180203 3

An increasing production of natural rubber (NR) products has led to major challenges in waste management. In this study, the degradation of rubber latex gloves in a mineral salt medium (MSM) using a bacterial consortium, a mixed culture of the selected bacteria and a pure culture were studied. The highest 18% weight loss of the rubber gloves were detected after incubated with the mixed culture. The increased viable cell counts over incubation time indicated that cells used rubber gloves as sole ...[more]

PMID: 29449176

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Project description:BackgroundMicrobial biodegradation of oil-hydrocarbons is one of the sustainable and cost-effective methods to remove petroleum spills from contaminated environments. The current study aimed to investigate the biodegradation abilities of three Fusarium isolates from oil reservoirs in Saudi Arabia. The novelty of the current work is that the biodegradation ability of these isolates was never tested against some natural hydrocarbons of variable compositions, such as Crude oil, and those of known components such as kerosene and diesel oils.MethodsThe isolates were treated with five selected hydrocarbons. The hydrocarbon tolerance test in solid and liquid media was performed. The scanning electron microscope (SEM) investigated the morphological changes of treated fungi. 2, 6-Dichlorophenol Indophenol (DCPIP), drop collapse, emulsification activity, and oil Spreading assays investigated the biodegradation ability. The amount of produced biosurfactants was measured, and their safety profile was estimated by the germination assay of tomato seeds.ResultsThe tolerance test showed enhanced fungal growth of all isolates, whereas the highest dose inhibition response (DIR) was 77% for Fusarium proliferatum treated with the used oil (p < 0.05). SEM showed morphological changes in all isolates. DCPIP results showed that used oil had the highest biodegradation by Fusarium verticillioides and Fusarium oxysporum. Mixed oil induced the highest effect in oil spreading, drop collapse, and emulsification assay caused by F. proliferatum. The highest recovery of biosurfactants was obtained by the solvent extraction method for F. verticillioides (4.6 g/L), F. proliferatum (4.22 g/L), and F. oxysporum (3.73 g/L). The biosurfactants produced by the three isolates stimulated tomato seeds' germination more than in control experiments.ConclusionThe current study suggested the possible oil-biodegradation activities induced by three Fusarium isolates from Riyadh, Saudi Arabia. The produced biosurfactants are not toxic against tomato seed germination, emphasizing their environmental sustainability. Further studies are required to investigate the mechanism of biodegradation activities and the chemical composition of the biosurfactants produced by these species.

Dataset Information

Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples.

Publications

Rubber gloves biodegradation by a consortium, mixed culture and pure culture isolated from soil samples.

Similar Datasets

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