Unknown

Dataset Information

0

Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis.


ABSTRACT:

Background

Precision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering).

Results

We used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 mutants exhibited a striking increase in tillering, combined with a reduced height, which could be rescued by application of the synthetic SL analog GR24. Striga germination assays and liquid chromatography-mass spectrometry analysis showed that root exudates of ccd7 mutants were also SL deficient.

Conclusions

Taken together, our results show the potential and feasibility of the use of the CRISPR/Cas9 system for targeted engineering of plant architecture and for elucidating the molecular underpinnings of architecture-related traits.

SUBMITTER: Butt H 

PROVIDER: S-EPMC6116466 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

Engineering plant architecture via CRISPR/Cas9-mediated alteration of strigolactone biosynthesis.

Butt Haroon H   Jamil Muhammad M   Wang Jian You JY   Al-Babili Salim S   Mahfouz Magdy M  

BMC plant biology 20180829 1


<h4>Background</h4>Precision plant genome engineering holds much promise for targeted improvement of crop traits via unprecedented single-base level control over the genetic material. Strigolactones (SLs) are a key determinant of plant architecture, known for their role in inhibiting shoot branching (tillering).<h4>Results</h4>We used CRISPR/Cas9 in rice (Oryza sativa) for targeted disruption of CAROTENOID CLEAVAGE DIOXYGENASE 7 (CCD7), which controls a key step in SL biosynthesis. The ccd7 muta  ...[more]

Similar Datasets

| S-EPMC6454688 | biostudies-literature
| S-EPMC4877446 | biostudies-other
| S-EPMC11156739 | biostudies-literature
| S-EPMC4670990 | biostudies-literature
| S-EPMC7588440 | biostudies-literature
| S-EPMC5030659 | biostudies-literature
| S-EPMC8494898 | biostudies-literature
| S-EPMC4720985 | biostudies-literature
| S-EPMC6341727 | biostudies-literature
| S-EPMC5363507 | biostudies-literature