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Spatiotemporal Control of TGF-? Signaling with Light.

ABSTRACT: Cells employ signaling pathways to make decisions in response to changes in their immediate environment. Transforming growth factor beta (TGF-?) is an important growth factor that regulates many cellular functions in development and disease. Although the molecular mechanisms of TGF-? signaling have been well studied, our understanding of this pathway is limited by the lack of tools that allow the control of TGF-? signaling with high spatiotemporal resolution. Here, we developed an optogenetic system (optoTGFBRs) that enables the precise control of TGF-? signaling in time and space. Using the optoTGFBRs system, we show that TGF-? signaling can be selectively and sequentially activated in single cells through the modulation of the pattern of light stimulations. By simultaneously monitoring the subcellular localization of TGF-? receptor and Smad2 proteins, we characterized the dynamics of TGF-? signaling in response to different patterns of blue light stimulations. The spatial and temporal precision of light control will make the optoTGFBRs system as a powerful tool for quantitative analyses of TGF-? signaling at the single cell level.

SUBMITTER: Li Y 

PROVIDER: S-EPMC6116903 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature

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Spatiotemporal Control of TGF-β Signaling with Light.

Li Yuchao Y   Lee Minji M   Kim Nury N   Wu Guoyu G   Deng Difan D   Kim Jin Man JM   Liu Xuedong X   Heo Won Do WD   Zi Zhike Z  

ACS synthetic biology 20180112 2

Cells employ signaling pathways to make decisions in response to changes in their immediate environment. Transforming growth factor beta (TGF-β) is an important growth factor that regulates many cellular functions in development and disease. Although the molecular mechanisms of TGF-β signaling have been well studied, our understanding of this pathway is limited by the lack of tools that allow the control of TGF-β signaling with high spatiotemporal resolution. Here, we developed an optogenetic sy  ...[more]