Dataset Information

De novo Transcriptome Characterization of Rhodomyrtus tomentosa Leaves and Identification of Genes Involved in ?/?-Pinene and ?-Caryophyllene Biosynthesis.

ABSTRACT: Plant-derived terpenes are effective in treating chronic dysentery, rheumatism, hepatitis, and hyperlipemia. Thus, understanding the molecular basis of terpene biosynthesis in some terpene-abundant Chinese medicinal plants is of great importance. Abundant in mono- and sesqui-terpenes, Rhodomyrtus tomentosa (Ait.) Hassk, an evergreen shrub belonging to the family Myrtaceae, is widely used as a traditional Chinese medicine. In this study, (+)-?-pinene and ?-caryophyllene were detected to be the two major components in the leaves of R. tomentosa, in which (+)-?-pinene is higher in the young leaves than in the mature leaves, whereas the distribution of ?-caryophyllene is opposite. Genome-wide transcriptome analysis of leaves identified 138 unigenes potentially involved in terpenoid biosynthesis. By integrating known biosynthetic pathways for terpenoids, 7 candidate genes encoding terpene synthase (RtTPS1-7) that potentially catalyze the last step in pinene and caryophyllene biosynthesis were further characterized. Sequence alignment analysis showed that RtTPS1, RtTPS3 and RtTPS4 do not contain typical N-terminal transit peptides (62-64aa), thus probably producing multiple isomers and enantiomers by terpenoid isomerization. Further enzyme activity in vitro confirmed that RtTPS1-4 mainly produce (+)-?-pinene and (+)-?-pinene, as well as small amounts of (-)-?-pinene and (-)-?-pinene with GPP, while RtTPS1 and RtTPS3 are also active with FPP, producing ?-caryophyllene, along with a smaller amount of ?-humulene. Our results deepen the understanding of molecular mechanisms of terpenes biosynthesis in Myrtaceae.

SUBMITTER: He SM

PROVIDER: S-EPMC6117411 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

De novo Transcriptome Characterization of Rhodomyrtus tomentosa Leaves and Identification of Genes Involved in α/β-Pinene and β-Caryophyllene Biosynthesis.

He Si-Mei SM Wang Xiao X Yang Sheng-Chao SC Dong Yang Y Zhao Qi-Ming QM Yang Jian-Li JL Cong Kun K Zhang Jia-Jin JJ Zhang Guang-Hui GH Wang Ying Y Fan Wei W

Frontiers in plant science 20180824

Plant-derived terpenes are effective in treating chronic dysentery, rheumatism, hepatitis, and hyperlipemia. Thus, understanding the molecular basis of terpene biosynthesis in some terpene-abundant Chinese medicinal plants is of great importance. Abundant in mono- and sesqui-terpenes, Rhodomyrtus tomentosa (Ait.) Hassk, an evergreen shrub belonging to the family Myrtaceae, is widely used as a traditional Chinese medicine. In this study, (+)-α-pinene and β-caryophyllene were detected to be ...[more]

PMID: 30197651

Similar Datasets

Project description:The formation of atmospheric cloud droplets due to secondary organic aerosol (SOA) particles is important for quantifying the Earth's radiative balance under future, possibly warmer, climates, yet is only poorly understood. While cloud activation may be parametrized using the surface tension depression that coincides with surfactant partitioning to the gas-droplet interface, the extent to which cloud activation is influenced by both the chemical structure and reactivity of the individual molecules comprising this surfactant pool is largely unknown. We report herein considerable differences in the surface tension depression of aqueous pendant droplets that contain synthetically prepared ozonolysis products derived from α-pinene and β-caryophyllene, the most abundant of the monoterpenes and sesquiterpenes, respectively, that are emitted over the planet's vast forest ecosystems. Oxidation products derived from β-caryophyllene were found to exhibit significantly higher surface activity than those prepared from α-pinene, with the critical supersaturation required for cloud droplet activation reduced by 50% for β-caryophyllene aldehyde at 1 mM. These considerable reductions in the critical supersaturation were found to coincide with free energies of adsorption that exceed ∼25 kJ/mol, or just one hydrogen bond equivalent, depending on the ammonium sulfate and oxidation product concentration in the solution. Additional experiments showed that aldehyde-containing oxidation products exist in equilibrium with hydrated forms in aqueous solution, which may modulate their bulk solubility and surface activity. Equilibration time scales on the order of 10-5 to 10-4 s calculated for micrometer-sized aerosol particles indicate instantaneous surface tension depression in the activation processes leading to cloud formation in the atmosphere. Our findings highlight the underlying importance of molecular structure and reactivity when considering cloud condensation activity in the presence of SOA particles.

Dataset Information

De novo Transcriptome Characterization of Rhodomyrtus tomentosa Leaves and Identification of Genes Involved in ?/?-Pinene and ?-Caryophyllene Biosynthesis.

Publications

<i>De novo</i> Transcriptome Characterization of <i>Rhodomyrtus tomentosa</i> Leaves and Identification of Genes Involved in α/β-Pinene and β-Caryophyllene Biosynthesis.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets