Chiral separation of diastereomers of the cyclic nonapeptides vasopressin and desmopressin by uniform field ion mobility mass spectrometry.

ABSTRACT: In this study ion mobility-mass spectrometry (IM-MS) is used to distinguish chiral diastereomers of the nonapeptides desmopressin and vasopressin. The differences in gas phase cross sectional area (ca. 2%) were sufficient to directly resolve the enantiomers present in a binary mixture. Results from computational modeling indicate that chiral recognition by IM-MS for nonapeptides is possible due to their diastereomer-specific conformations adopted in the gas-phase, namely a compact ring-tail conformer specific to the l-diastereomer forms.

SUBMITTER: Phillips ST 

PROVIDER: S-EPMC6118329 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature