Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.
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ABSTRACT:
SUBMITTER: Vogl T
PROVIDER: S-EPMC6123417 | biostudies-literature | 2018 Sep
REPOSITORIES: biostudies-literature
Publications
Engineered bidirectional promoters enable rapid multi-gene co-expression optimization.
Vogl Thomas T Kickenweiz Thomas T Pitzer Julia J Sturmberger Lukas L Weninger Astrid A Biggs Bradley W BW Köhler Eva-Maria EM Baumschlager Armin A Fischer Jasmin Elgin JE Hyden Patrick P Wagner Marlies M Baumann Martina M Borth Nicole N Geier Martina M Ajikumar Parayil Kumaran PK Glieder Anton A
Nature communications 20180904 1
Numerous synthetic biology endeavors require well-tuned co-expression of functional components for success. Classically, monodirectional promoters (MDPs) have been used for such applications, but MDPs are limited in terms of multi-gene co-expression capabilities. Consequently, there is a pressing need for new tools with improved flexibility in terms of genetic circuit design, metabolic pathway assembly, and optimization. Here, motivated by nature's use of bidirectional promoters (BDPs) as a solu ...[more]
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