Dataset Information

Investigation of Physicho-chemical Properties and Characterization of Produced Biosurfactant by Selected Indigenous Oil-degrading Bacterium.

ABSTRACT: Due to the amphipathic properties of biosurfactants which act on surfaces and interfaces interest by a variety of industries such as cosmetic, pharmaceutical, bioremediation and petroleum-related industries has recently increased.Detection of a high-efficiency biosurfactant producer using preliminary screening methods from soil contaminated with crude oil was carried at the Microbiology Laboratory at Shahid Beheshti University, Tehran, Iran in 2013. Then after characterization of some physico-chemical properties of produced biosurfactant and production optimization conditions, processes of purification and complete identification were done.Pseudomonas aeruginosa sp. ZN was selected as high-efficiency biosurfactant producing strain from soil contaminated with oil from Ahvaz City, Khuzestan Province, southern Iran. The biosurfactant production in modified BH2 culture medium supplemented with 1% n-hexadecane occurred during exponential phase resulting in a reduction surface tension from 70 to 29 mN/m. Strain ZN produced biosurfactant with different properties to other Pseudomonas reported. These characterizations included continued production at C/N ratio range of 10-40; the produced biosurfactant could not separate stable emulsion of span-80-kerosene: Tween-80-distilled water (30:70) within 24 h. The produced biosurfactants were able to increase hydrophobicity of bacterial cell to 55%. Recovery of biosurfactants from cell-free supernatant was performed with acid precipitation and ammonium sulfate precipitation. Chemical analysis such as spraying techniques on developed TLC plate and staining methods of supernatant indicated that produced biosurfactants were glycolipids, characterized by ESI-MS analysis of extracted product as di-rhamnolipids.Ability of this strain to produce biosurfactant in the presence of cooked oil and n-hexadecane make it an optimistic candidate for biodegradation of some derivatives of crude oil and food industry.

SUBMITTER: Najmi Z

PROVIDER: S-EPMC6123595 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Investigation of Physicho-chemical Properties and Characterization of Produced Biosurfactant by Selected Indigenous Oil-degrading Bacterium.

Najmi Ziba Z Ebrahimipour Gholamhossein G Franzetti Andrea A Banat Ibrahim Mohamed IM

Iranian journal of public health 20180801 8

<h4>Background</h4>Due to the amphipathic properties of biosurfactants which act on surfaces and interfaces interest by a variety of industries such as cosmetic, pharmaceutical, bioremediation and petroleum-related industries has recently increased.<h4>Methods</h4>Detection of a high-efficiency biosurfactant producer using preliminary screening methods from soil contaminated with crude oil was carried at the Microbiology Laboratory at Shahid Beheshti University, Tehran, Iran in 2013. Then after ...[more]

PMID: 30186787

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Project description:Surfactants can reduce the interfacial surface tension between two immiscible liquids making them a desirable component for various industrial applications. However, the toxic nature of chemical surfactants brought immense attention towards biosurfactants. Being biodegradable, biosurfactants are eco-friendly and considered safer for different commercial uses. This study focused on the production of biosurfactant from an oil-degrading bacteria and its functional efficacy for prospective industrial applications. Here, a promising oil-tolerant strain, Bacillus velezensis S2 was isolated from oil contaminated sites which showed >50% degradation of convoluted crude oil within 28 days in comparison to a control. The isolate was then found to produce an excellent surface-active compound with an emulsification index of 67.30 ± 0.8% and could reduce the surface tension up to 36.86 ± 0.36 mN m-1. It also manifested a critical micelle concentration of 45 mg L-1 while reducing the surface tension from 72 to 30 mN m-1. When extracting biosurfactant from isolated bacteria, ethyl acetate extraction showed 1.5 times greater efficacy than chloroform : methanol extraction. The purified biosurfactant was characterized using TLC, 1H NMR, 13C NMR, FTIR, elemental analyses and spectrophotometric techniques leading to its identification as a rhamnolipid. The stability of produced biosurfactant at higher temperature (up to 180 °C) was determined by thermal analysis, endorsing its application in high temperature reservoir conditions. Additionally, the extracted biosurfactant showed excellent foaming efficacy with insignificant antibacterial and cytotoxic responses, which indicates their potential application in cleaning and cosmetics industries. Thus, the present study outlines a bi-functional novel isolate Bacillus velezensis S2 which could play a significant role in oil remediation from the environment as well as serve as a potential source of non-toxic and eco-friendly biosurfactants for various industrial applications.

Dataset Information

Investigation of Physicho-chemical Properties and Characterization of Produced Biosurfactant by Selected Indigenous Oil-degrading Bacterium.

Publications

Investigation of Physicho-chemical Properties and Characterization of Produced Biosurfactant by Selected Indigenous Oil-degrading Bacterium.

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