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Fluorimetric detection of reserpine in mouse serum through online post-column electrochemical derivatization.

ABSTRACT: A novel method combining high-performance liquid chromatography with online post-column electrochemical derivatization and fluorescence detection was established for the detection of reserpine in mouse serum. Reserpine separation was conducted using a C18 column with 5?mM H3PO4 and acetonitrile (55/45, v/v) as eluent. Reserpine was then electro-oxidized into a strongly fluorescent compound using an electrolytic cell device. Detection parameters, such as potential and fluorescence wavelength, were optimized. The linearity of the proposed method ranged from 0.01 to 5.0?mg?l-1 with a correlation coefficient of 0.9997. The limit of qualification (S/N?=?10) and limit of detection (S/N?=?3) were 9.7 and 2.9?µg?l-1, respectively. Resperine recoveries from spiked blank and drug-treated mouse serum samples ranged from 92.0 to 115%.

SUBMITTER: Chen N

PROVIDER: S-EPMC6124075 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Fluorimetric detection of reserpine in mouse serum through online post-column electrochemical derivatization.

Chen Ning N Li Weixia W Wu Shuchao S Zhu Yan Y

Royal Society open science 20180815 8

A novel method combining high-performance liquid chromatography with online post-column electrochemical derivatization and fluorescence detection was established for the detection of reserpine in mouse serum. Reserpine separation was conducted using a C18 column with 5 mM H<sub>3</sub>PO<sub>4</sub> and acetonitrile (55/45, v/v) as eluent. Reserpine was then electro-oxidized into a strongly fluorescent compound using an electrolytic cell device. Detection parameters, such as potential and fluore ...[more]

PMID: 30224984

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