Unknown

Dataset Information

0

A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples.


ABSTRACT: A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC50) of the strip in the detection of fish extract samples was confirmed to be 1.86 µg kg-1, and the limit of detection value was 0.62 µg kg-1. For intra-assay and inter-assay reproducibility, recoveries of HES-spiked samples ranged from 86.3% to 92.3% and 85.8% to 93.4%, coefficients of variation were 2.91-4.64% and 4.24-5.17%, respectively. High-performance liquid chromatography was employed to confirm the performance of the strip. The strip test takes less than 10 min, and thus provides a repaid method for on-site detection of HES residues.

SUBMITTER: Yang X 

PROVIDER: S-EPMC6124078 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

altmetric image

Publications

A lateral flow immunochromatographic strip test for rapid detection of hexoestrol in fish samples.

Yang Xingdong X   Sun Zhongke Z   Tian Fengshou F   Jia Guochao G   Yang Jifei J   Hu Xiaofei X  

Royal Society open science 20180829 8


A lateral flow immunochromatographic strip test was developed for rapid and sensitive on-site detection of hexoestrol (HES) residues in fish samples with colloidal gold labelling of the anti-HES monoclonal antibody. The strip is composed of a sample pad, a conjugate reagent pad, an absorbent pad and a test membrane containing a control line and a test line. The sensitivity (half inhibitory concentration, IC<sub>50</sub>) of the strip in the detection of fish extract samples was confirmed to be 1  ...[more]

Similar Datasets

| S-EPMC6598930 | biostudies-literature
| S-EPMC7540878 | biostudies-literature
| S-EPMC8153588 | biostudies-literature
| S-EPMC5738603 | biostudies-literature
| S-EPMC6017486 | biostudies-literature
| S-EPMC10313708 | biostudies-literature
| S-EPMC5450700 | biostudies-literature
| S-EPMC8761541 | biostudies-literature
| S-EPMC4609312 | biostudies-literature
| S-EPMC6917642 | biostudies-literature