Ontology highlight
ABSTRACT:
SUBMITTER: Mancuso JE
PROVIDER: S-EPMC6124747 | biostudies-literature | 2018
REPOSITORIES: biostudies-literature
Mancuso Jordan E JE Jayaraman Anjana A Ristenpart William D WD
PloS one 20180905 9
Centrifugation is the primary preparation step for isolating red blood cells (RBCs) from whole blood, including for use in studies focused on transduction of adenosine triphosphate (ATP), an important vasodilatory signaling molecule. Despite the wide use of centrifugation, little work has focused on how the centrifugation itself affects release of ATP from RBCs prior to subsequent experimentation. Here we report that both the centrifugation force and duration have a pronounced impact on the conc ...[more]